Sort By
Publication Year
Deposit Date
Title
Type
Access
Publication Year
2012 (1)
2011 (1)
2010 (1)
2009 (1)
2007 (3)
2005 (3)
2004 (1)
2002 (4)
2001 (1)
2000 (3)
Version
M-Rank
M23 (5)
M24 (1)

Konstantinov, Kosana

Link to this page

Authority KeyName Variants
9f904186-21ee-4463-991f-3480f6c65764
  • Konstantinov, Kosana (19)
Projects

Author's Bibliography

Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD)

Mataruga, Milan; Isajev, Vasilije; Konstantinov, Kosana; Mladenović-Drinić, Snežana; Ballian, Dalibor

(Ferdinand Berger Soehne, Horn, 2012)

TY  - JOUR
AU  - Mataruga, Milan
AU  - Isajev, Vasilije
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
AU  - Ballian, Dalibor
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/431
AB  - Proteins as gene markers of tolerance to drought in Austrian pine (Pinus nigra ARNOLD). - Phyton (Horn, Austria) 52 (2): 263-280, with 8 figures. The aim of the research was to determine the variability of protein content in seeds and seedlings at the level of the studied provenances, populations and open-pollinated trees. Studies have included 40 trees that originated from 5 provenances in the Balkan Peninsula. Within each provenance, seeds were collected from five trees growing in extremely harsh habitat (rocky terrain population) and three trees growing in a favorable habitat (control population). The specifics of the studied provenances, populations and open-pollinated trees have been confirmed in the analysis of salt soluble protein with molecular weight from 12.42 kDa to 240kDa. Variability and stability of proteins were observed on the seed, and then on 9-day-old seedlings obtained on a 4% sucrose solution (drought conditions) and distilled water (control). The analysis of seed protein complex shows the most significant differences between the analyzed provenances, whereas the differences between populations and habitat conditions are insignificant. Also, there is a high intra- and inter- open-polinated trees variability of the analyzed proteins. At the same time, protein complex in the seedlings obtained in drought and controlled conditions points to the differences among the analyzed populations, ie. it shows a different gene expression depending on the differences in habitats where the seed were collected (rocky-control). This research is important for practical application in the production of seeds and planting materials which could be used for afforestation in extreme habitats.
PB  - Ferdinand Berger Soehne, Horn
T2  - Phyton-Annales Rei Botanicae
T1  - Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD)
VL  - 52
IS  - 2
SP  - 263
EP  - 280
ER  - 
@article{
author = "Mataruga, Milan and Isajev, Vasilije and Konstantinov, Kosana and Mladenović-Drinić, Snežana and Ballian, Dalibor",
year = "2012",
url = "http://rik.mrizp.rs/handle/123456789/431",
abstract = "Proteins as gene markers of tolerance to drought in Austrian pine (Pinus nigra ARNOLD). - Phyton (Horn, Austria) 52 (2): 263-280, with 8 figures. The aim of the research was to determine the variability of protein content in seeds and seedlings at the level of the studied provenances, populations and open-pollinated trees. Studies have included 40 trees that originated from 5 provenances in the Balkan Peninsula. Within each provenance, seeds were collected from five trees growing in extremely harsh habitat (rocky terrain population) and three trees growing in a favorable habitat (control population). The specifics of the studied provenances, populations and open-pollinated trees have been confirmed in the analysis of salt soluble protein with molecular weight from 12.42 kDa to 240kDa. Variability and stability of proteins were observed on the seed, and then on 9-day-old seedlings obtained on a 4% sucrose solution (drought conditions) and distilled water (control). The analysis of seed protein complex shows the most significant differences between the analyzed provenances, whereas the differences between populations and habitat conditions are insignificant. Also, there is a high intra- and inter- open-polinated trees variability of the analyzed proteins. At the same time, protein complex in the seedlings obtained in drought and controlled conditions points to the differences among the analyzed populations, ie. it shows a different gene expression depending on the differences in habitats where the seed were collected (rocky-control). This research is important for practical application in the production of seeds and planting materials which could be used for afforestation in extreme habitats.",
publisher = "Ferdinand Berger Soehne, Horn",
journal = "Phyton-Annales Rei Botanicae",
title = "Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD)",
volume = "52",
number = "2",
pages = "263-280"
}
Mataruga, M., Isajev, V., Konstantinov, K., Mladenović-Drinić, S.,& Ballian, D. (2012). Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD).
Phyton-Annales Rei BotanicaeFerdinand Berger Soehne, Horn., 52(2), 263-280.
Mataruga M, Isajev V, Konstantinov K, Mladenović-Drinić S, Ballian D. Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD). Phyton-Annales Rei Botanicae. 2012;52(2):263-280
Mataruga Milan, Isajev Vasilije, Konstantinov Kosana, Mladenović-Drinić Snežana, Ballian Dalibor, "Proteins as Gene Markers of Tolerance to Drought in Austrian Pine (Pinus nigra ARNOLD)" 52, no. 2 (2012):263-280
1
1

The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje

Babić, Milosav; Anđelković, Violeta; Mladenović-Drinić, Snežana; Konstantinov, Kosana

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2011)

TY  - JOUR
AU  - Babić, Milosav
AU  - Anđelković, Violeta
AU  - Mladenović-Drinić, Snežana
AU  - Konstantinov, Kosana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/394
AB  - Broad genetic variability of starting biological material is crucial prerequisite for the successful breeding program. Maize Research Institute, owning Gene bank with more than 6,000 accessions consisted of the local and introduced collection, has the opportunity for the investigation both fundamental genetic processes, pre-breeding and breeding for commercial purposes. To search for new sources of maize drought tolerance Gene bank accessions were scored visually on stay-green phenotype and total appearance. More than 50 genotypes have been identified as a potential source for drought tolerance. Besides breeding on improved storage protein quality decreasing of phytate content in kernel is also under the consideration, because the most phosphorous is bound in phytate. In breeding program it is desirable to have genotypes with higher content of available phosphorous (P) not bound in phytate. Among 60 analyzed populations from Gene bank only one has been determined to have very low phytate content and will be used in maize breeding program on low phytate in kernel. Process of maize breeding, to get high yielding hybrids, begins by the genetic variability determination of starting breeding material, either populations or selected inbred lines. Genetic divergence of parental inbred lines is main step to get high heterotic effect in yield after crossing. Use of different molecular markers allowed cluster analysis by use of UPGMA methods and select high number of genotypes to be included in crossing process.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje
VL  - 56
IS  - 2
SP  - 155
EP  - 163
ER  - 
@article{
author = "Babić, Milosav and Anđelković, Violeta and Mladenović-Drinić, Snežana and Konstantinov, Kosana",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/394",
abstract = "Broad genetic variability of starting biological material is crucial prerequisite for the successful breeding program. Maize Research Institute, owning Gene bank with more than 6,000 accessions consisted of the local and introduced collection, has the opportunity for the investigation both fundamental genetic processes, pre-breeding and breeding for commercial purposes. To search for new sources of maize drought tolerance Gene bank accessions were scored visually on stay-green phenotype and total appearance. More than 50 genotypes have been identified as a potential source for drought tolerance. Besides breeding on improved storage protein quality decreasing of phytate content in kernel is also under the consideration, because the most phosphorous is bound in phytate. In breeding program it is desirable to have genotypes with higher content of available phosphorous (P) not bound in phytate. Among 60 analyzed populations from Gene bank only one has been determined to have very low phytate content and will be used in maize breeding program on low phytate in kernel. Process of maize breeding, to get high yielding hybrids, begins by the genetic variability determination of starting breeding material, either populations or selected inbred lines. Genetic divergence of parental inbred lines is main step to get high heterotic effect in yield after crossing. Use of different molecular markers allowed cluster analysis by use of UPGMA methods and select high number of genotypes to be included in crossing process.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje",
volume = "56",
number = "2",
pages = "155-163"
}
Babić, M., Anđelković, V., Mladenović-Drinić, S.,& Konstantinov, K. (2011). The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje.
MaydicaMaydica-Ist Sper Cerealicoltur, Bergamo., 56(2), 155-163.
Babić M, Anđelković V, Mladenović-Drinić S, Konstantinov K. The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje. Maydica. 2011;56(2):155-163
Babić Milosav, Anđelković Violeta, Mladenović-Drinić Snežana, Konstantinov Kosana, "The conventional and contemporary technologies in maize (Zea mays L) breeding at Maize Research Institut Zemun Polje" 56, no. 2 (2011):155-163
10

Ethics in scientific results application: Gene and life forms patenting

Konstantinov, Kosana; Mladenović-Drinić, Snežana; Anđelković, Violeta; Babić, Milosav

(Društvo genetičara Srbije, Beograd, 2010)

TY  - JOUR
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
AU  - Anđelković, Violeta
AU  - Babić, Milosav
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/333
AB  - The remarkable development and application of new genetic technologies over the past decades has been accompanied by profound changes in the way in which research is commercialized in the life sciences. As results, new varieties of commercially grown crops with improved or new traits are developed. Many thousands of patents which assert rights over DNA sequences have been granted to researchers across the public and private sector. The effects of many of these patents are extensive, because inventors who assert rights over DNA sequences obtain protection on all uses of the sequences. Extremely valuable to breeders in the national agricultural research system is the ability to genotype their collections to get a clear picture of their diversity and how diversity could be enhanced through sharing and access to global collections. The issue of the eligibility for patenting of DNA sequences needs to be reopened. Patents that assert rights over DNA sequences and their uses are, in some cases, supportable, but in others, should be treated with great caution. Rights over DNA sequences as research tools should be discouraged. That the best way to discourage the award of such patents is by stringent application of the criteria for patenting, particularly utility. A more equitable, ethically - based food and agricultural system must incorporate concern for three accepted global goals: improved well being, protection of the environment and improved public health (particular point food from GMO). To mitigate conflict one of the approach to solve problem is ethical and truthful label of GM food, because consumers have a right to choose whether to eat genetically modified foods or not. Interesting examples and risks as consequences of free availability of genetic resources utilization, its transformation, patenting of 'new' organism and selling it back to the genetic resource owner are presented. Society has obligations to raise levels of nutrition and standards living by all respect to ethics at each step.
AB  - Razvoj novih biotehnologija zasnovanih na tehnologiji rekombinantne DNK, definisanih kao genetičko inženjerstvo, izazvao je značajne promene u načinu primene i komercijalizacije naučnih rezultata dobijenih u oblasti osnovnih istraživanja, posebno u poljoprivredi, medicini i zaštiti životne sredine. Na hiljade patenata koji daju pravo na vlasništvo nad DNA sekvencama/genima je odobreno istraživačima u privatnom i javnom sektoru. Korišćenjem patentiranih gena, koji kontrolišu važne osobine (otpornost prema bolestima na pr.) stvorene su i uvedene u proizvodnju nove sorte i hibridi najznačajnijih gajenih biljnih vrsta kao što su kukuruz, soja, pirinač kao i mnoge ukrasne biljke. Istraživači koji su vlasnici patenta gena imaju pravo na patentnu zaštitu i transgenih biljaka koje poseduju taj gen. Od posebnog značaja za istraživače u poljoprivredi, posebno u oblasti genetike i oplemenjivanja je mogućnost, korišćenjem genskih proba, karakterizacije genotipova i dobijanje pouzdanih podataka o genetičkoj varijabilnosti, naročito u korišćenju kako u sopstvenim programima tako i u razmeni biološkog materijala sa drugim kolekcijama. Slobodna dostupnost tih proba je jedan od preduslova. Pitanje opravdanosti patentiranja sekvenci DNA/gena i njihovog korišćenja je neophodno ponovo otvoriti jer su patenti u nekom slučaju opravdani a unekom moraju da budu oprezno razmatrani.. Patentno pravo na sekvencu DNA/gen kao eksperimentalni materijal nije opravdano i treba ga obeshrabrivati. Najbolji način za ograničavanje zloupotrebe je strogo primena kriterijuma za patentiranje, posebno za pravo korišćenja patenata. Mnogo pravedniji, na etičkim principima zasnovan sistem proizvodnje hrane mora da ima ugrađena tri globalna cilja: poboljšanje kvaliteta života, zaštitu životne sredine i obezbeđenje zdravlja populacije sa akcentom na korišćenje hrane proizvedene od GMO. Jedan od pristupa ostvarenju ovih ciljeva je etičko i istinito obeležavanje hrane od GMO, jer potrošači imaju pravo izbora da li žele da koriste genetički modifikovanu hranu ili ne. Interesantan je primer i rizici koji proizilaze iz neograničene dostupnosti i korišćenja genetičkih resursa, njihova transformacija korišćenjem genetičkog inženjerstva, patentiranje 'novog' genotipa - organizma a zatim njegova prodaja vlasniku korišćenog originalnog genotipa. Društvo ima obavezu podizanja nivoa kvaliteta hrane I standard življenja uz puno poštovanje etika na svakom stepenu razvoja. U radu su navedeni neki od slučajeva patentiranja koji ne zadovoljavaju osnovne etičke norme.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Ethics in scientific results application: Gene and life forms patenting
T1  - Etika u korišćenju naučnih rezultata - patentiranje gena i organizama
VL  - 42
IS  - 1
SP  - 193
EP  - 208
DO  - 10.2298/GENSR1001193K
ER  - 
@article{
author = "Konstantinov, Kosana and Mladenović-Drinić, Snežana and Anđelković, Violeta and Babić, Milosav",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/333",
abstract = "The remarkable development and application of new genetic technologies over the past decades has been accompanied by profound changes in the way in which research is commercialized in the life sciences. As results, new varieties of commercially grown crops with improved or new traits are developed. Many thousands of patents which assert rights over DNA sequences have been granted to researchers across the public and private sector. The effects of many of these patents are extensive, because inventors who assert rights over DNA sequences obtain protection on all uses of the sequences. Extremely valuable to breeders in the national agricultural research system is the ability to genotype their collections to get a clear picture of their diversity and how diversity could be enhanced through sharing and access to global collections. The issue of the eligibility for patenting of DNA sequences needs to be reopened. Patents that assert rights over DNA sequences and their uses are, in some cases, supportable, but in others, should be treated with great caution. Rights over DNA sequences as research tools should be discouraged. That the best way to discourage the award of such patents is by stringent application of the criteria for patenting, particularly utility. A more equitable, ethically - based food and agricultural system must incorporate concern for three accepted global goals: improved well being, protection of the environment and improved public health (particular point food from GMO). To mitigate conflict one of the approach to solve problem is ethical and truthful label of GM food, because consumers have a right to choose whether to eat genetically modified foods or not. Interesting examples and risks as consequences of free availability of genetic resources utilization, its transformation, patenting of 'new' organism and selling it back to the genetic resource owner are presented. Society has obligations to raise levels of nutrition and standards living by all respect to ethics at each step., Razvoj novih biotehnologija zasnovanih na tehnologiji rekombinantne DNK, definisanih kao genetičko inženjerstvo, izazvao je značajne promene u načinu primene i komercijalizacije naučnih rezultata dobijenih u oblasti osnovnih istraživanja, posebno u poljoprivredi, medicini i zaštiti životne sredine. Na hiljade patenata koji daju pravo na vlasništvo nad DNA sekvencama/genima je odobreno istraživačima u privatnom i javnom sektoru. Korišćenjem patentiranih gena, koji kontrolišu važne osobine (otpornost prema bolestima na pr.) stvorene su i uvedene u proizvodnju nove sorte i hibridi najznačajnijih gajenih biljnih vrsta kao što su kukuruz, soja, pirinač kao i mnoge ukrasne biljke. Istraživači koji su vlasnici patenta gena imaju pravo na patentnu zaštitu i transgenih biljaka koje poseduju taj gen. Od posebnog značaja za istraživače u poljoprivredi, posebno u oblasti genetike i oplemenjivanja je mogućnost, korišćenjem genskih proba, karakterizacije genotipova i dobijanje pouzdanih podataka o genetičkoj varijabilnosti, naročito u korišćenju kako u sopstvenim programima tako i u razmeni biološkog materijala sa drugim kolekcijama. Slobodna dostupnost tih proba je jedan od preduslova. Pitanje opravdanosti patentiranja sekvenci DNA/gena i njihovog korišćenja je neophodno ponovo otvoriti jer su patenti u nekom slučaju opravdani a unekom moraju da budu oprezno razmatrani.. Patentno pravo na sekvencu DNA/gen kao eksperimentalni materijal nije opravdano i treba ga obeshrabrivati. Najbolji način za ograničavanje zloupotrebe je strogo primena kriterijuma za patentiranje, posebno za pravo korišćenja patenata. Mnogo pravedniji, na etičkim principima zasnovan sistem proizvodnje hrane mora da ima ugrađena tri globalna cilja: poboljšanje kvaliteta života, zaštitu životne sredine i obezbeđenje zdravlja populacije sa akcentom na korišćenje hrane proizvedene od GMO. Jedan od pristupa ostvarenju ovih ciljeva je etičko i istinito obeležavanje hrane od GMO, jer potrošači imaju pravo izbora da li žele da koriste genetički modifikovanu hranu ili ne. Interesantan je primer i rizici koji proizilaze iz neograničene dostupnosti i korišćenja genetičkih resursa, njihova transformacija korišćenjem genetičkog inženjerstva, patentiranje 'novog' genotipa - organizma a zatim njegova prodaja vlasniku korišćenog originalnog genotipa. Društvo ima obavezu podizanja nivoa kvaliteta hrane I standard življenja uz puno poštovanje etika na svakom stepenu razvoja. U radu su navedeni neki od slučajeva patentiranja koji ne zadovoljavaju osnovne etičke norme.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Ethics in scientific results application: Gene and life forms patenting, Etika u korišćenju naučnih rezultata - patentiranje gena i organizama",
volume = "42",
number = "1",
pages = "193-208",
doi = "10.2298/GENSR1001193K"
}
Konstantinov, K., Mladenović-Drinić, S., Anđelković, V.,& Babić, M. (2010). Etika u korišćenju naučnih rezultata - patentiranje gena i organizama.
GenetikaDruštvo genetičara Srbije, Beograd., 42(1), 193-208.
https://doi.org/10.2298/GENSR1001193K
Konstantinov K, Mladenović-Drinić S, Anđelković V, Babić M. Etika u korišćenju naučnih rezultata - patentiranje gena i organizama. Genetika. 2010;42(1):193-208
Konstantinov Kosana, Mladenović-Drinić Snežana, Anđelković Violeta, Babić Milosav, "Etika u korišćenju naučnih rezultata - patentiranje gena i organizama" 42, no. 1 (2010):193-208,
https://doi.org/10.2298/GENSR1001193K .
3
1
2

Use of non parametric statistics in estimation of genotypes stability

Delić, Nenad; Stanković, Goran; Konstantinov, Kosana

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2009)

TY  - JOUR
AU  - Delić, Nenad
AU  - Stanković, Goran
AU  - Konstantinov, Kosana
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/277
AB  - The Mostly used, classical parametric approaches for an analysis of genotype x environment interaction are based on several assumptions: normality of: the distribution, homogeneity of variances, additivity. If some of mentioned assumptions are not fulfilled, the validity of these methods may be questionable. By use of nonparametric methods, which are simple and easy for analysis, all of the mentioned assumptions are avoided. in this paper we used several of non parametric techniques for analysis of genotype x environment interaction for grain yield of 24 maize (Zea mays Q hybrids through two locations in two years. Values of the stability measures shown that hybrids with the highest grain yield in the majority of cases were not the most stable. So as the value of the relative deviation in relation to the average rank classified the lowest yielding (4.9 t ha(-1)) genotype PR36B08 as the most stable genotype (S-i((3)) = 0.12). The interrelations of numerical values among methods, indicating the genotype x environment interaction obtained in this study, are different than the relation given by HUHN (1996), hence the order is as follows: van der Laan and de Kroon>KubingerHildebrand The Values of mean differences among ranks over different environments (S-i((1))) were ranged froin 2.33 to 14.83. The values of variance among ranks (Si(2)) varied from 3.33 to 154.92, while values of the relative deviations in relation to the average rank (Si 6)) ranged from 0.12 to 15.55.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Use of non parametric statistics in estimation of genotypes stability
VL  - 54
IS  - 2-3
SP  - 155
EP  - 160
ER  - 
@article{
author = "Delić, Nenad and Stanković, Goran and Konstantinov, Kosana",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/277",
abstract = "The Mostly used, classical parametric approaches for an analysis of genotype x environment interaction are based on several assumptions: normality of: the distribution, homogeneity of variances, additivity. If some of mentioned assumptions are not fulfilled, the validity of these methods may be questionable. By use of nonparametric methods, which are simple and easy for analysis, all of the mentioned assumptions are avoided. in this paper we used several of non parametric techniques for analysis of genotype x environment interaction for grain yield of 24 maize (Zea mays Q hybrids through two locations in two years. Values of the stability measures shown that hybrids with the highest grain yield in the majority of cases were not the most stable. So as the value of the relative deviation in relation to the average rank classified the lowest yielding (4.9 t ha(-1)) genotype PR36B08 as the most stable genotype (S-i((3)) = 0.12). The interrelations of numerical values among methods, indicating the genotype x environment interaction obtained in this study, are different than the relation given by HUHN (1996), hence the order is as follows: van der Laan and de Kroon>KubingerHildebrand The Values of mean differences among ranks over different environments (S-i((1))) were ranged froin 2.33 to 14.83. The values of variance among ranks (Si(2)) varied from 3.33 to 154.92, while values of the relative deviations in relation to the average rank (Si 6)) ranged from 0.12 to 15.55.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Use of non parametric statistics in estimation of genotypes stability",
volume = "54",
number = "2-3",
pages = "155-160"
}
Delić, N., Stanković, G.,& Konstantinov, K. (2009). Use of non parametric statistics in estimation of genotypes stability.
MaydicaMaydica-Ist Sper Cerealicoltur, Bergamo., 54(2-3), 155-160.
Delić N, Stanković G, Konstantinov K. Use of non parametric statistics in estimation of genotypes stability. Maydica. 2009;54(2-3):155-160
Delić Nenad, Stanković Goran, Konstantinov Kosana, "Use of non parametric statistics in estimation of genotypes stability" 54, no. 2-3 (2009):155-160
7
10

Molecular genetics: Step by step implementation in maize breeding

Konstantinov, Kosana; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2007)

TY  - JOUR
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/150
AB  - Efficiency in plant breeding is determined primarily by the ability to screen for genetic polymorphism, productivity and yield stability early in program. Dependent on the knowledge about the biochemical bases of the trait and nature of its genetic control, trait could be modified either through mutagenesis of genes controlling it or through the transfer of already existing mutant genes, controlling desired trait to different plant genotypes by classic crossing. Objective of this report is to present partly results on the investigation of the possibilities to apply ionizing radiations (fast neutrons, γ -rays) and chemical mutagens (EI, iPMS, EMS, ENU) to get maize and wheat mutants with increased amount and improved protein quality. Besides this approach in mutation breeding, results on the very early investigation of biochemical background of opaque -2 mutation including use of coupled cell - free RNA and protein synthesis containing components from both wild and opaque - 2 maize genotypes (chromatin, RNA polymerase, microsomall fraction, protein bodies) will be presented. Partial results on opaque - 2 gene incorporation in different genetic background are reviewed. Part of report is dealing with different classes of molecular markers (proteins, RFLP, AFLP, RAPD, and SSR) application in maize genome polymorphism investigation. Besides application of different molecular markers classes in the investigation of heterosis phenomena they are useful in biochemical pathway of important traits control determination as well.
AB  - Efikasnost procesa oplemenjivanja je primarno determinisana mogućnostima utvrđivanja genetičkog polimorfizma, produktivnosti i stabilnosti prinosa početnog materijala. Poznavanjem biohemijske osnove genetičke kontrole željene osobine moguće je izvršiti poželjnu promenu ili modifikaciju ili indukovanjem mutacija ili unošenjem već postojećih poznatih gena nosioca mutacija klasičnim procesom ukrštanja sa različitim genotipovima. U radu je dat parcijalni pregled indukovanja mutacija kod pšenice i kukuruza, dobijenih korišćenjem različitih vrsta jonizujućeg zračenja (brzi neutroni, γ -zraci) ili hemijskih mutagena (EI, iPMS, EMS, ENU). U drugom delu su prikazani neki od rezultata unošenja opaque-2 gena u različite genetičke osnove kukuruza primenom klasičnih metoda oplemenjivanja. Istovremeno su navedeni rezultati istraživanja biohemijske osnove opaque2 mutacije, uključujući korišćenje ukopčanog bezćelijskog sistema za sintezu RNK i proteina u prisustvu različitih ćelijskih komponenata (hromatin, RNK polimeraza, mikrozomalna frakcija, proteinska telašca) izolovanih iz endosperma normalnog genotipa i opaque-2 mutanta kukuruza. Deo rada predstavlja pregled rezultata istraživanja u oblasti molekularnog oplemenjivanja - korišćenja različitih klasa molekularnih markera (RFLP, AFLP, RAPD, SSR) u izučavanju genetičkog polimorfizma kukuruza. Dat je i prikaz rezultata ispitivanja molekularne osnove heterozisa kod kukuruza korišćenjem molekularnih markera.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular genetics: Step by step implementation in maize breeding
T1  - Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza
VL  - 39
IS  - 2
SP  - 139
EP  - 154
DO  - 10.2298/GENSR0702139K
ER  - 
@article{
author = "Konstantinov, Kosana and Mladenović-Drinić, Snežana",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/150",
abstract = "Efficiency in plant breeding is determined primarily by the ability to screen for genetic polymorphism, productivity and yield stability early in program. Dependent on the knowledge about the biochemical bases of the trait and nature of its genetic control, trait could be modified either through mutagenesis of genes controlling it or through the transfer of already existing mutant genes, controlling desired trait to different plant genotypes by classic crossing. Objective of this report is to present partly results on the investigation of the possibilities to apply ionizing radiations (fast neutrons, γ -rays) and chemical mutagens (EI, iPMS, EMS, ENU) to get maize and wheat mutants with increased amount and improved protein quality. Besides this approach in mutation breeding, results on the very early investigation of biochemical background of opaque -2 mutation including use of coupled cell - free RNA and protein synthesis containing components from both wild and opaque - 2 maize genotypes (chromatin, RNA polymerase, microsomall fraction, protein bodies) will be presented. Partial results on opaque - 2 gene incorporation in different genetic background are reviewed. Part of report is dealing with different classes of molecular markers (proteins, RFLP, AFLP, RAPD, and SSR) application in maize genome polymorphism investigation. Besides application of different molecular markers classes in the investigation of heterosis phenomena they are useful in biochemical pathway of important traits control determination as well., Efikasnost procesa oplemenjivanja je primarno determinisana mogućnostima utvrđivanja genetičkog polimorfizma, produktivnosti i stabilnosti prinosa početnog materijala. Poznavanjem biohemijske osnove genetičke kontrole željene osobine moguće je izvršiti poželjnu promenu ili modifikaciju ili indukovanjem mutacija ili unošenjem već postojećih poznatih gena nosioca mutacija klasičnim procesom ukrštanja sa različitim genotipovima. U radu je dat parcijalni pregled indukovanja mutacija kod pšenice i kukuruza, dobijenih korišćenjem različitih vrsta jonizujućeg zračenja (brzi neutroni, γ -zraci) ili hemijskih mutagena (EI, iPMS, EMS, ENU). U drugom delu su prikazani neki od rezultata unošenja opaque-2 gena u različite genetičke osnove kukuruza primenom klasičnih metoda oplemenjivanja. Istovremeno su navedeni rezultati istraživanja biohemijske osnove opaque2 mutacije, uključujući korišćenje ukopčanog bezćelijskog sistema za sintezu RNK i proteina u prisustvu različitih ćelijskih komponenata (hromatin, RNK polimeraza, mikrozomalna frakcija, proteinska telašca) izolovanih iz endosperma normalnog genotipa i opaque-2 mutanta kukuruza. Deo rada predstavlja pregled rezultata istraživanja u oblasti molekularnog oplemenjivanja - korišćenja različitih klasa molekularnih markera (RFLP, AFLP, RAPD, SSR) u izučavanju genetičkog polimorfizma kukuruza. Dat je i prikaz rezultata ispitivanja molekularne osnove heterozisa kod kukuruza korišćenjem molekularnih markera.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular genetics: Step by step implementation in maize breeding, Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza",
volume = "39",
number = "2",
pages = "139-154",
doi = "10.2298/GENSR0702139K"
}
Konstantinov, K.,& Mladenović-Drinić, S. (2007). Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza.
GenetikaDruštvo genetičara Srbije, Beograd., 39(2), 139-154.
https://doi.org/10.2298/GENSR0702139K
Konstantinov K, Mladenović-Drinić S. Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza. Genetika. 2007;39(2):139-154
Konstantinov Kosana, Mladenović-Drinić Snežana, "Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza" 39, no. 2 (2007):139-154,
https://doi.org/10.2298/GENSR0702139K .
2

Proteins in seed and seedlings of selected Austrian pine (Pinus nigra Arnold) trees as genetic markers tolerant to drought

Mataruga, Milan; Isajev, Vasilije; Konstantinov, Kosana; Mladenović-Drinić, Snežana; Daničić, Vanja

(Društvo genetičara Srbije, Beograd, 2007)

TY  - JOUR
AU  - Mataruga, Milan
AU  - Isajev, Vasilije
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
AU  - Daničić, Vanja
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/176
AB  - A precondition necessary for creation and selection of genotypes tolerant of stress conditions is a study of physiological, biochemical and molecular bases of their adaptive reaction to stress. The study includes 40 lines of free pollination originating from 5 provenances: Sutjeska, Višegrad, Tara, Teslić and Durmitor (B&H, Serbia and Montenegro). Two populations were selected from each provenance, i.e.: 5 lines of free pollination represent the population growing on the cliffs and 3 lines of free pollination represent the population growing at the best site of Austrian pine. Specific characteristics of the studied provenances, populations, and free pollination lines were confirmed by the analyses in the salt-soluble proteins. The identical protein composition was proved in a small number of cases in the replicates of the same free pollination line, which indicates a high intra-line variability, which can be the result of the effect of father, as well as of heterozygosity of mother trees. The analyses of protein composition of seed showed considerable differences at provenance level. Inter-line, population and provenance differences, and also, the interaction between the origin and drought factor, were recorded for 9-day-old seedlings germinated in induced drought conditions and in normal conditions.
AB  - Preduslov za stvaranje i selekciju tolerantnih genotipova na stresne uslove je proučavanje fiziološke, biohemijske i molekularne osnove njihove adaptivne reakcije na stres. Istraživanjima je obuhvaćeno 40 linija crnog bora slobodnog oprašivanja koje potiču iz 5 provenijencija: Sutjeska, Višegrad, Tara, Teslić i Durmitor (BiH, Srbije i Crna Gora). U svakoj provenijenciji izdvojene su dvije populacije i to: 5 linija slobodnog oprašivanja predstavlja populaciju sa stijena i 3 linije slobodnog oprašivanja populaciju na najboljem staništu crnog bora. Specifičnost ispitivanih provenijencija, populacija, kao i linija slobodnog oprašivanja dokazana je putem analiza u solima rastvorljivih proteina. Identičan proteinski sastav u ponavljanjima iste linije slobodnog oprašivanja je dokazan u malom broju slučajeva, što govori u prilog velike unutarlinijske varijabilnosti koja može biti posledica uticaja oca, kao i heterozigotnosti materinskih stabala. U analizama proteinskog sastava sjemena konstatovane su najznačajnije razlike na nivou provenijencija. Kod klijavaca starih 9 dana, isklijalih u uslovima indukovane suše i u standardnim uslovima, utvrđene su međulinijske, populacione i provenijenične razlike, kao i značajne interakcije porekla i stresnog faktora suše.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Proteins in seed and seedlings of selected Austrian pine (Pinus nigra Arnold) trees as genetic markers tolerant to drought
T1  - Proteini u semenu i klijancima austrijskog bora (Pinus nigra Arnold) kao genetički markeri tolerantnosti na sušu
VL  - 39
IS  - 2
SP  - 259
EP  - 272
DO  - 10.2298/GENSR0702259M
ER  - 
@article{
author = "Mataruga, Milan and Isajev, Vasilije and Konstantinov, Kosana and Mladenović-Drinić, Snežana and Daničić, Vanja",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/176",
abstract = "A precondition necessary for creation and selection of genotypes tolerant of stress conditions is a study of physiological, biochemical and molecular bases of their adaptive reaction to stress. The study includes 40 lines of free pollination originating from 5 provenances: Sutjeska, Višegrad, Tara, Teslić and Durmitor (B&H, Serbia and Montenegro). Two populations were selected from each provenance, i.e.: 5 lines of free pollination represent the population growing on the cliffs and 3 lines of free pollination represent the population growing at the best site of Austrian pine. Specific characteristics of the studied provenances, populations, and free pollination lines were confirmed by the analyses in the salt-soluble proteins. The identical protein composition was proved in a small number of cases in the replicates of the same free pollination line, which indicates a high intra-line variability, which can be the result of the effect of father, as well as of heterozygosity of mother trees. The analyses of protein composition of seed showed considerable differences at provenance level. Inter-line, population and provenance differences, and also, the interaction between the origin and drought factor, were recorded for 9-day-old seedlings germinated in induced drought conditions and in normal conditions., Preduslov za stvaranje i selekciju tolerantnih genotipova na stresne uslove je proučavanje fiziološke, biohemijske i molekularne osnove njihove adaptivne reakcije na stres. Istraživanjima je obuhvaćeno 40 linija crnog bora slobodnog oprašivanja koje potiču iz 5 provenijencija: Sutjeska, Višegrad, Tara, Teslić i Durmitor (BiH, Srbije i Crna Gora). U svakoj provenijenciji izdvojene su dvije populacije i to: 5 linija slobodnog oprašivanja predstavlja populaciju sa stijena i 3 linije slobodnog oprašivanja populaciju na najboljem staništu crnog bora. Specifičnost ispitivanih provenijencija, populacija, kao i linija slobodnog oprašivanja dokazana je putem analiza u solima rastvorljivih proteina. Identičan proteinski sastav u ponavljanjima iste linije slobodnog oprašivanja je dokazan u malom broju slučajeva, što govori u prilog velike unutarlinijske varijabilnosti koja može biti posledica uticaja oca, kao i heterozigotnosti materinskih stabala. U analizama proteinskog sastava sjemena konstatovane su najznačajnije razlike na nivou provenijencija. Kod klijavaca starih 9 dana, isklijalih u uslovima indukovane suše i u standardnim uslovima, utvrđene su međulinijske, populacione i provenijenične razlike, kao i značajne interakcije porekla i stresnog faktora suše.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Proteins in seed and seedlings of selected Austrian pine (Pinus nigra Arnold) trees as genetic markers tolerant to drought, Proteini u semenu i klijancima austrijskog bora (Pinus nigra Arnold) kao genetički markeri tolerantnosti na sušu",
volume = "39",
number = "2",
pages = "259-272",
doi = "10.2298/GENSR0702259M"
}
Mataruga, M., Isajev, V., Konstantinov, K., Mladenović-Drinić, S.,& Daničić, V. (2007). Proteini u semenu i klijancima austrijskog bora (Pinus nigra Arnold) kao genetički markeri tolerantnosti na sušu.
GenetikaDruštvo genetičara Srbije, Beograd., 39(2), 259-272.
https://doi.org/10.2298/GENSR0702259M
Mataruga M, Isajev V, Konstantinov K, Mladenović-Drinić S, Daničić V. Proteini u semenu i klijancima austrijskog bora (Pinus nigra Arnold) kao genetički markeri tolerantnosti na sušu. Genetika. 2007;39(2):259-272
Mataruga Milan, Isajev Vasilije, Konstantinov Kosana, Mladenović-Drinić Snežana, Daničić Vanja, "Proteini u semenu i klijancima austrijskog bora (Pinus nigra Arnold) kao genetički markeri tolerantnosti na sušu" 39, no. 2 (2007):259-272,
https://doi.org/10.2298/GENSR0702259M .
7

Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves

Radenović, Čedomir; Konstantinov, Kosana; Delić, Nenad; Stanković, Goran

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2007)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Konstantinov, Kosana
AU  - Delić, Nenad
AU  - Stanković, Goran
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/167
AB  - The initial idea of this paper was a hypothesis that upright leaves of maize inbred lines were characteristic for an efficient photosynthetic model and that as such were very desirable in increasing the number of plants per area unit (plant density) in the process of contemporary breeding and seed production. The application of a semi in vivo photosynthetic bioluminescence method (leaf segment is still alive and all process are active), suitable for the efficiency estimation of the model, verified the hypothesis. Obtained photosynthetic properties of observed upright leaves of maize inbred lines were based on the effect and photosynthetic characteristics of thermal processes of delayed chlorophyll fluorescence (DF) occurring in their thylakoid membranes. The temperature dependence of the delayed chlorophyll fluorescence intensity, phase transition (critical temperatures) and activation energy are the principal photosynthetic parameters of the thermal processes in the thylakoid membrane. Based on obtained photosynthetic and bioluminescence properties it is possible to select maize inbred lines having upright leaves that are resistant and tolerant to high and very high temperatures, as well as to drought. The selected maize inbred lines could be considered as the efficient photosynthetic model and as such could be used in the improvement of the contemporary maize breeding and seed production.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves
VL  - 52
IS  - 3
SP  - 347
EP  - 356
ER  - 
@article{
author = "Radenović, Čedomir and Konstantinov, Kosana and Delić, Nenad and Stanković, Goran",
year = "2007",
url = "http://rik.mrizp.rs/handle/123456789/167",
abstract = "The initial idea of this paper was a hypothesis that upright leaves of maize inbred lines were characteristic for an efficient photosynthetic model and that as such were very desirable in increasing the number of plants per area unit (plant density) in the process of contemporary breeding and seed production. The application of a semi in vivo photosynthetic bioluminescence method (leaf segment is still alive and all process are active), suitable for the efficiency estimation of the model, verified the hypothesis. Obtained photosynthetic properties of observed upright leaves of maize inbred lines were based on the effect and photosynthetic characteristics of thermal processes of delayed chlorophyll fluorescence (DF) occurring in their thylakoid membranes. The temperature dependence of the delayed chlorophyll fluorescence intensity, phase transition (critical temperatures) and activation energy are the principal photosynthetic parameters of the thermal processes in the thylakoid membrane. Based on obtained photosynthetic and bioluminescence properties it is possible to select maize inbred lines having upright leaves that are resistant and tolerant to high and very high temperatures, as well as to drought. The selected maize inbred lines could be considered as the efficient photosynthetic model and as such could be used in the improvement of the contemporary maize breeding and seed production.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves",
volume = "52",
number = "3",
pages = "347-356"
}
Radenović, Č., Konstantinov, K., Delić, N.,& Stanković, G. (2007). Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves.
MaydicaMaydica-Ist Sper Cerealicoltur, Bergamo., 52(3), 347-356.
Radenović Č, Konstantinov K, Delić N, Stanković G. Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves. Maydica. 2007;52(3):347-356
Radenović Čedomir, Konstantinov Kosana, Delić Nenad, Stanković Goran, "Photosynthetic and bioluminescence properties of maize inbred lines with upright leaves" 52, no. 3 (2007):347-356
4
4

Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers

Bauer, Iva; Mladenović-Drinić, Snežana; Filipović, Milomir; Konstantinov, Kosana

(Društvo genetičara Srbije, Beograd, 2005)

TY  - JOUR
AU  - Bauer, Iva
AU  - Mladenović-Drinić, Snežana
AU  - Filipović, Milomir
AU  - Konstantinov, Kosana
PY  - 2005
UR  - http://rik.mrizp.rs/handle/123456789/99
AB  - Knowledge of maize germplasm genetic diversity is important for planning breeding programmes, germplasm conservation per se etc. Genetic variability of maize hybrids grown in the fields is also very important because genetic uniformity implies risks of genetic vulnerability to stress factors and can cause great losts in yield. Early maturing maize hybrids are characterized by shorter vegetation period and they are grown in areas with shorter vegetation season. Because of different climatic conditions in these areas lines and hybrids are developed with different features in respect to drought resistance and disease resistance. The objective of our study was to characterize set of early maturing maize hybrids with protein and RAPD markers and to compare this clasification with their pedigree information. RAPD markers gave significantly higher rate of polymorphism than protein markers. Better corelation was found among pedigree information and protein markers.
AB  - Poznavanje genetičke varijubilnosti germplazme kukuruza je važno zbog planiranja programa selekcije, konzervacije germplazme per se. Genetička uniformnost uključuje rizik od velikih gubitaka u prinosima zbog izuzetne osetljivosti ovakvih useva na stresne faktore sredine. Rani hibridi se odlikuju kraćim vegetacionim periodom i namenjeni su za gajenje u područjima sa kraćom vegetacionom sezonom. Zbog različitih klimatskih uslova u ovim područjima stvorene su linije i hibridi sa različitim osobinama u pogledu otpornosti na susu i bolesti lista, stabla i klipa. Cilj rada je bila karakterizacija ranih hibrida različitog poreklu primenom proteinskih i RAPD markera. U ovom radu RAPD markeri su pokazali znatno viši nivo polimorfizma, dok su proteinski markeri pokazali bolje slaganje sa pedigre podacima ispitivanih hibrida.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers
T1  - Genetička karakterizacija ranih hibrida kukuruza (Zea mays L) primenom proteinskih i RAPD markera
VL  - 37
IS  - 3
SP  - 235
EP  - 243
DO  - 10.2298/GENSR0503235B
ER  - 
@article{
author = "Bauer, Iva and Mladenović-Drinić, Snežana and Filipović, Milomir and Konstantinov, Kosana",
year = "2005",
url = "http://rik.mrizp.rs/handle/123456789/99",
abstract = "Knowledge of maize germplasm genetic diversity is important for planning breeding programmes, germplasm conservation per se etc. Genetic variability of maize hybrids grown in the fields is also very important because genetic uniformity implies risks of genetic vulnerability to stress factors and can cause great losts in yield. Early maturing maize hybrids are characterized by shorter vegetation period and they are grown in areas with shorter vegetation season. Because of different climatic conditions in these areas lines and hybrids are developed with different features in respect to drought resistance and disease resistance. The objective of our study was to characterize set of early maturing maize hybrids with protein and RAPD markers and to compare this clasification with their pedigree information. RAPD markers gave significantly higher rate of polymorphism than protein markers. Better corelation was found among pedigree information and protein markers., Poznavanje genetičke varijubilnosti germplazme kukuruza je važno zbog planiranja programa selekcije, konzervacije germplazme per se. Genetička uniformnost uključuje rizik od velikih gubitaka u prinosima zbog izuzetne osetljivosti ovakvih useva na stresne faktore sredine. Rani hibridi se odlikuju kraćim vegetacionim periodom i namenjeni su za gajenje u područjima sa kraćom vegetacionom sezonom. Zbog različitih klimatskih uslova u ovim područjima stvorene su linije i hibridi sa različitim osobinama u pogledu otpornosti na susu i bolesti lista, stabla i klipa. Cilj rada je bila karakterizacija ranih hibrida različitog poreklu primenom proteinskih i RAPD markera. U ovom radu RAPD markeri su pokazali znatno viši nivo polimorfizma, dok su proteinski markeri pokazali bolje slaganje sa pedigre podacima ispitivanih hibrida.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers, Genetička karakterizacija ranih hibrida kukuruza (Zea mays L) primenom proteinskih i RAPD markera",
volume = "37",
number = "3",
pages = "235-243",
doi = "10.2298/GENSR0503235B"
}
Bauer, I., Mladenović-Drinić, S., Filipović, M.,& Konstantinov, K. (2005). Genetička karakterizacija ranih hibrida kukuruza (Zea mays L) primenom proteinskih i RAPD markera.
GenetikaDruštvo genetičara Srbije, Beograd., 37(3), 235-243.
https://doi.org/10.2298/GENSR0503235B
Bauer I, Mladenović-Drinić S, Filipović M, Konstantinov K. Genetička karakterizacija ranih hibrida kukuruza (Zea mays L) primenom proteinskih i RAPD markera. Genetika. 2005;37(3):235-243
Bauer Iva, Mladenović-Drinić Snežana, Filipović Milomir, Konstantinov Kosana, "Genetička karakterizacija ranih hibrida kukuruza (Zea mays L) primenom proteinskih i RAPD markera" 37, no. 3 (2005):235-243,
https://doi.org/10.2298/GENSR0503235B .
9

Genetically modified food: Benefits and risks

Mladenović-Drinić, Snežana; Drinić, Goran; Konstantinov, Kosana

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2005)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Drinić, Goran
AU  - Konstantinov, Kosana
PY  - 2005
UR  - http://rik.mrizp.rs/handle/123456789/102
AB  - The variety of foods consumed by humans has changed greatly over the centuries, altering the balance of nutrients in the diet. More consumer-oriented GM products including nutritionally enhanced foods, such as fats and oils with reduced amounts of saturated fatty acid, potatoes that absorb less fat in frying, lower calorie sugar, reduced allergenic and antinutritional compounds will soon appear on market. Consumers have a fundamental right to know what they are eating, and is it safe. All food derived from genetically modified plants must be evaluated for food safety before they can enter into the food supply. The health effect of genetically modified food depends of specific content of food itself and could be either potentionally beneficial, for example food with higher content of iron vitamins or elimination of alergent from food or harmful where alergent or toxin is introduced in food. There are no evidence that foods made from GM crops are less safe than traditional foods. With the increasing availability of genetically modified plant products it has become necessary to develop techniques for identification of such products in order to follow labeling requirements and national regulations.
AB  - Tokom vekova raznovrsnost hrane koja se koristi za ishranu ljudi se značajno menjala, menjajući i balans i vrednost hranjivih materija koje se unose. Veliki broj proizvoda dobijen iz genetički modifikovanih biljka je već na tržištu uključen u lanac ishrane. Vise potrošačima orijentisani genetički modifikovani proizvodi uključujući proizvode sa promenjenim ili poboljšanim kvalitetom; masti i ulja sa redukovanim količinama zasićenih masnih kiselina; krompir koji absorbuje manje masti pri prženju; nisko kalorični šećer; proizvodi sa redukovanim alergentima i antihranjivim materijama će se uskoro pojaviti na tržištu. Genetički modifikovani proizvodi sadrže sastojke koji nikad ranije nisu bili deo lanca ishrane i potrošači imaju pravo da znaju šta jedu i da li je hrana bezbedna. Uticaj hrane dobijene od genetički modifikovanih biljaka na zdravlje ljudi zavisi od specifičnog sadržaja i može biti potencijalno korisno ako se radi o proizvodu sa povećanim sadržajem na primer gvožđa, vitamina, antioksidanata ili smanjenim sadržajem zasićenih masnih kiselina i potencijalno štetno ako se modifikacijama unese alergen ili toksin. Svaki proizvod dobijen od genetički modifikovanih biljaka podleže rigoroznom ispitivanju bezbednosti pre nego što se odobri za upotrebu. Za sada nema dokaza da je hrana dobijena od genetički modifikovanih biljaka manje bezbedna od tradicionalne hrane. Tehnike za identifikaciju genetičkih modifikacija su razvijene a većina država je razvila sopstvenu regulativu za obeležavanje ovih proizvoda.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Genetically modified food: Benefits and risks
T1  - Hrana proizvedena od GMO - koristi i rizici
VL  - 66
IS  - 5
SP  - 125
EP  - 137
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Drinić, Goran and Konstantinov, Kosana",
year = "2005",
url = "http://rik.mrizp.rs/handle/123456789/102",
abstract = "The variety of foods consumed by humans has changed greatly over the centuries, altering the balance of nutrients in the diet. More consumer-oriented GM products including nutritionally enhanced foods, such as fats and oils with reduced amounts of saturated fatty acid, potatoes that absorb less fat in frying, lower calorie sugar, reduced allergenic and antinutritional compounds will soon appear on market. Consumers have a fundamental right to know what they are eating, and is it safe. All food derived from genetically modified plants must be evaluated for food safety before they can enter into the food supply. The health effect of genetically modified food depends of specific content of food itself and could be either potentionally beneficial, for example food with higher content of iron vitamins or elimination of alergent from food or harmful where alergent or toxin is introduced in food. There are no evidence that foods made from GM crops are less safe than traditional foods. With the increasing availability of genetically modified plant products it has become necessary to develop techniques for identification of such products in order to follow labeling requirements and national regulations., Tokom vekova raznovrsnost hrane koja se koristi za ishranu ljudi se značajno menjala, menjajući i balans i vrednost hranjivih materija koje se unose. Veliki broj proizvoda dobijen iz genetički modifikovanih biljka je već na tržištu uključen u lanac ishrane. Vise potrošačima orijentisani genetički modifikovani proizvodi uključujući proizvode sa promenjenim ili poboljšanim kvalitetom; masti i ulja sa redukovanim količinama zasićenih masnih kiselina; krompir koji absorbuje manje masti pri prženju; nisko kalorični šećer; proizvodi sa redukovanim alergentima i antihranjivim materijama će se uskoro pojaviti na tržištu. Genetički modifikovani proizvodi sadrže sastojke koji nikad ranije nisu bili deo lanca ishrane i potrošači imaju pravo da znaju šta jedu i da li je hrana bezbedna. Uticaj hrane dobijene od genetički modifikovanih biljaka na zdravlje ljudi zavisi od specifičnog sadržaja i može biti potencijalno korisno ako se radi o proizvodu sa povećanim sadržajem na primer gvožđa, vitamina, antioksidanata ili smanjenim sadržajem zasićenih masnih kiselina i potencijalno štetno ako se modifikacijama unese alergen ili toksin. Svaki proizvod dobijen od genetički modifikovanih biljaka podleže rigoroznom ispitivanju bezbednosti pre nego što se odobri za upotrebu. Za sada nema dokaza da je hrana dobijena od genetički modifikovanih biljaka manje bezbedna od tradicionalne hrane. Tehnike za identifikaciju genetičkih modifikacija su razvijene a većina država je razvila sopstvenu regulativu za obeležavanje ovih proizvoda.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Genetically modified food: Benefits and risks, Hrana proizvedena od GMO - koristi i rizici",
volume = "66",
number = "5",
pages = "125-137"
}
Mladenović-Drinić, S., Drinić, G.,& Konstantinov, K. (2005). Hrana proizvedena od GMO - koristi i rizici.
Journal of Scientific Agricultural ResearchSavez poljoprivrednih inženjera i tehničara, Beograd., 66(5), 125-137.
Mladenović-Drinić S, Drinić G, Konstantinov K. Hrana proizvedena od GMO - koristi i rizici. Journal of Scientific Agricultural Research. 2005;66(5):125-137
Mladenović-Drinić Snežana, Drinić Goran, Konstantinov Kosana, "Hrana proizvedena od GMO - koristi i rizici" 66, no. 5 (2005):125-137

Biotechnology in plant breeding: New knowledge in resolving old problems

Konstantinov, Kosana; Mladenović-Drinić, Snežana; Drinić, Goran

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2005)

TY  - JOUR
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
AU  - Drinić, Goran
PY  - 2005
UR  - http://rik.mrizp.rs/handle/123456789/106
AB  - Molecular biology and molecular genetics development, particularly development of recombinant DNA technology and genetic manipulation in vitro combined with cell and tissue culture ended by the specific method biotechnology., different than classic definition of it. This biotechnology as combination of biology, biochemistry, molecular biology, genetic engineering and computer science, enabled completely new approaches in plant on the diseases resistance, pesticide tolerance, increasing of genetic potential in the higher yield of better quality. Recombinant DNA technology has been developed after discovery possibility of bacterial endonucleasses to cut foreign DNA at specific nucleotide sequences and ligation of complementary DNA fragment ends producing recombinant DNA molecule. This technology is background of new biotechnology method by which genes controlling useful traits could be transferred between different organisms what has as end product genetically modified organisms (GMO). Both biotechnology and classical methods of plant breeding have the same goal but use of biotechnology has a lot of advantage, including transfer of single genes of interest, originated even from different evolutionary category. By classic breeding technology there is possibility to make combination combine only between different genotypes of the same plant species or between much closed genotypes.
AB  - Razvoj molekularne biologije i molekularne genetike posebno tehnologije rekombinantne DNK uz istovremeno usavršavanje tehnologija koje omogućavaju manipulacije u bezćelijskom sistemu kao što su kultura ćelija i tkiva rezultirao je u formiranju oblasti biotehnologije sa specifičnim osobinama u odnosu na klasične definicije. Integracijom najpropulzivnijih naučnih disciplina kao što su biologija, biohemija, molekularna biologija, genetika hemijski inženjering i informatika otvorene su neograničene mogućnosti i izazovi u sprečavanju bolesti humane populacije, biljaka i životinja kontroli insekata bez pesticida, povećanju produktivnosti stoke, povećanju proizvodnje i poboljšanju kvaliteta hrane i zaštite životne sredine. Primena tehnologije rekombinatne DNK ima nekoliko prednosti u odnosu na tradicionalno oplemenjivanje. Genetičke modifikacije biljaka kao i klasično oplemenjivanje imaju isti cilj, dobijanje biljaka sa poboljšanim svojstvima. Klasičnim metodama oplemenjivanja prenosi se kompletan set gena iz roditeljskih linija u novo potomstvo. Time se ne prenosi samo jedan ili dva željena gena već stotine drugih gena, neki poželjni, a neki ne. Takođe tradicionalne metode oplemenjivanja se zasnivaju na prenosu genetičkog materijala između jedinki iste ili veoma bliske vrste. Primenom biotehnologije moguće je preneti jedan ili vise poželjnih gena iz bilo koje evolucione kategorije u istu ili drugu kategoriju organizma i na taj način stvoriti genetički modifikovane organizme (GMO) sa željenim osobinama. U radu će biti dat pregled stanja u oblasti primene biotehnologije u svetu i kod nas.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Biotechnology in plant breeding: New knowledge in resolving old problems
T1  - Biotehnologija u oplemenjivanju biljaka - nova znanja u rešavanju starih problema
VL  - 66
IS  - 5
SP  - 35
EP  - 46
ER  - 
@article{
author = "Konstantinov, Kosana and Mladenović-Drinić, Snežana and Drinić, Goran",
year = "2005",
url = "http://rik.mrizp.rs/handle/123456789/106",
abstract = "Molecular biology and molecular genetics development, particularly development of recombinant DNA technology and genetic manipulation in vitro combined with cell and tissue culture ended by the specific method biotechnology., different than classic definition of it. This biotechnology as combination of biology, biochemistry, molecular biology, genetic engineering and computer science, enabled completely new approaches in plant on the diseases resistance, pesticide tolerance, increasing of genetic potential in the higher yield of better quality. Recombinant DNA technology has been developed after discovery possibility of bacterial endonucleasses to cut foreign DNA at specific nucleotide sequences and ligation of complementary DNA fragment ends producing recombinant DNA molecule. This technology is background of new biotechnology method by which genes controlling useful traits could be transferred between different organisms what has as end product genetically modified organisms (GMO). Both biotechnology and classical methods of plant breeding have the same goal but use of biotechnology has a lot of advantage, including transfer of single genes of interest, originated even from different evolutionary category. By classic breeding technology there is possibility to make combination combine only between different genotypes of the same plant species or between much closed genotypes., Razvoj molekularne biologije i molekularne genetike posebno tehnologije rekombinantne DNK uz istovremeno usavršavanje tehnologija koje omogućavaju manipulacije u bezćelijskom sistemu kao što su kultura ćelija i tkiva rezultirao je u formiranju oblasti biotehnologije sa specifičnim osobinama u odnosu na klasične definicije. Integracijom najpropulzivnijih naučnih disciplina kao što su biologija, biohemija, molekularna biologija, genetika hemijski inženjering i informatika otvorene su neograničene mogućnosti i izazovi u sprečavanju bolesti humane populacije, biljaka i životinja kontroli insekata bez pesticida, povećanju produktivnosti stoke, povećanju proizvodnje i poboljšanju kvaliteta hrane i zaštite životne sredine. Primena tehnologije rekombinatne DNK ima nekoliko prednosti u odnosu na tradicionalno oplemenjivanje. Genetičke modifikacije biljaka kao i klasično oplemenjivanje imaju isti cilj, dobijanje biljaka sa poboljšanim svojstvima. Klasičnim metodama oplemenjivanja prenosi se kompletan set gena iz roditeljskih linija u novo potomstvo. Time se ne prenosi samo jedan ili dva željena gena već stotine drugih gena, neki poželjni, a neki ne. Takođe tradicionalne metode oplemenjivanja se zasnivaju na prenosu genetičkog materijala između jedinki iste ili veoma bliske vrste. Primenom biotehnologije moguće je preneti jedan ili vise poželjnih gena iz bilo koje evolucione kategorije u istu ili drugu kategoriju organizma i na taj način stvoriti genetički modifikovane organizme (GMO) sa željenim osobinama. U radu će biti dat pregled stanja u oblasti primene biotehnologije u svetu i kod nas.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Biotechnology in plant breeding: New knowledge in resolving old problems, Biotehnologija u oplemenjivanju biljaka - nova znanja u rešavanju starih problema",
volume = "66",
number = "5",
pages = "35-46"
}
Konstantinov, K., Mladenović-Drinić, S.,& Drinić, G. (2005). Biotehnologija u oplemenjivanju biljaka - nova znanja u rešavanju starih problema.
Journal of Scientific Agricultural ResearchSavez poljoprivrednih inženjera i tehničara, Beograd., 66(5), 35-46.
Konstantinov K, Mladenović-Drinić S, Drinić G. Biotehnologija u oplemenjivanju biljaka - nova znanja u rešavanju starih problema. Journal of Scientific Agricultural Research. 2005;66(5):35-46
Konstantinov Kosana, Mladenović-Drinić Snežana, Drinić Goran, "Biotehnologija u oplemenjivanju biljaka - nova znanja u rešavanju starih problema" 66, no. 5 (2005):35-46

Biotechnology in maize breeding

Mladenović-Drinić, Snežana; Ignjatović-Micić, Dragana; Erić, Iva; Anđelković, Violeta; Jelovac, Dražen; Konstantinov, Kosana

(Društvo genetičara Srbije, Beograd, 2004)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Ignjatović-Micić, Dragana
AU  - Erić, Iva
AU  - Anđelković, Violeta
AU  - Jelovac, Dražen
AU  - Konstantinov, Kosana
PY  - 2004
UR  - http://rik.mrizp.rs/handle/123456789/65
AB  - Maize is one of the most important economic crops and the best studied and most tractable genetic system among monocots. The development of biotechnology has led to a great increase in our knowledge of maize genetics and understanding of the structure and behaviour of maize genomes. Conventional breeding practices can now be complemented by a number of new and powerful techniques. Some of these often referred to as molecular methods, enable scientists to see the layout of the entire genome of any organism and to select plants with preferred characteristics by "reading" at the molecular level, saving precious time and resources. DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. Application of molecular markers for genetic studies of maize include: assessment of genetic variability and characterization of germ plasm, identification and fingerprinting of genotypes, estimation of genetic distance, detection of monogamic and quantitative trait loci, marker assisted selection, identification of sequence of useful candidate genes, etc. The development of high-density molecular maps which has been facilitated by PCR-based markers, have made the mapping and tagging of almost any trait possible and serve as bases for marker assisted selection. Sequencing of maize genomes would help to elucidate gene function, gene regulation and their expression. Modern biotechnology also includes an array of tools for introducing or deieting a particular gene or genes to produce plants with novel traits. Development of informatics and biotechnology are resulted in bioinformatic as well as in expansion of microarrey technique. Modern biotechnologies could complement and improve the efficiency of traditional selection and breeding techniques to enhance agricultural productivity.
AB  - Kukuruz je jedan od ekonomski najznačajnijih useva i model sistem za genetička ispitivanja kod monokotila. Razvoj biotehnologije je omogućio bolje razumevanje strukture i funkcije genoma kukuruza a konvencionalno oplemenjivanje je dopunjeno novim i moćnim tehnikama. Neke od njih omogućavaju naučnicima da sagledaju strukturu celog genoma i odaberu biljke s poželjnim svojstvima na molekularnom nivou, štedeći vreme i resurse. Primena molekularnih markera uključuje ispitivanje genetičke varijabilnosti i karakterizaciju germplazme; identifikaciju gena koji kontrolišu agronomski važne osobine; selekciju pomoću markera. Sekvencioniranje genoma kukuruza pomaže rasvetljavanju funkcije, regulacije i ekspresije gena. Moderna biotehnologija uključuje seriju tehnika koje omogućavaju prenos gena iz drugih organizama ili deaktivaciju postojećih gena i stvaranje genotipova sa novim osobinama. Razvoj informatike i biotehnologije rezultirao je u stvaranju bioinformatike i omogućio je širu primenu mikroarrey tehnike. Moderna biotehnologija može da dopuni i poboljša efikasnost klasičnog oplemenjivanja u cilju stvaranja visokorodnih genotipova kukuruza otpornih na bolesti i stres.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Biotechnology in maize breeding
T1  - Biotehnologija u oplemenjivanju kukuruza
VL  - 36
IS  - 2
SP  - 93
EP  - 109
DO  - 10.2298/GENSR0402093M
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Ignjatović-Micić, Dragana and Erić, Iva and Anđelković, Violeta and Jelovac, Dražen and Konstantinov, Kosana",
year = "2004",
url = "http://rik.mrizp.rs/handle/123456789/65",
abstract = "Maize is one of the most important economic crops and the best studied and most tractable genetic system among monocots. The development of biotechnology has led to a great increase in our knowledge of maize genetics and understanding of the structure and behaviour of maize genomes. Conventional breeding practices can now be complemented by a number of new and powerful techniques. Some of these often referred to as molecular methods, enable scientists to see the layout of the entire genome of any organism and to select plants with preferred characteristics by "reading" at the molecular level, saving precious time and resources. DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. Application of molecular markers for genetic studies of maize include: assessment of genetic variability and characterization of germ plasm, identification and fingerprinting of genotypes, estimation of genetic distance, detection of monogamic and quantitative trait loci, marker assisted selection, identification of sequence of useful candidate genes, etc. The development of high-density molecular maps which has been facilitated by PCR-based markers, have made the mapping and tagging of almost any trait possible and serve as bases for marker assisted selection. Sequencing of maize genomes would help to elucidate gene function, gene regulation and their expression. Modern biotechnology also includes an array of tools for introducing or deieting a particular gene or genes to produce plants with novel traits. Development of informatics and biotechnology are resulted in bioinformatic as well as in expansion of microarrey technique. Modern biotechnologies could complement and improve the efficiency of traditional selection and breeding techniques to enhance agricultural productivity., Kukuruz je jedan od ekonomski najznačajnijih useva i model sistem za genetička ispitivanja kod monokotila. Razvoj biotehnologije je omogućio bolje razumevanje strukture i funkcije genoma kukuruza a konvencionalno oplemenjivanje je dopunjeno novim i moćnim tehnikama. Neke od njih omogućavaju naučnicima da sagledaju strukturu celog genoma i odaberu biljke s poželjnim svojstvima na molekularnom nivou, štedeći vreme i resurse. Primena molekularnih markera uključuje ispitivanje genetičke varijabilnosti i karakterizaciju germplazme; identifikaciju gena koji kontrolišu agronomski važne osobine; selekciju pomoću markera. Sekvencioniranje genoma kukuruza pomaže rasvetljavanju funkcije, regulacije i ekspresije gena. Moderna biotehnologija uključuje seriju tehnika koje omogućavaju prenos gena iz drugih organizama ili deaktivaciju postojećih gena i stvaranje genotipova sa novim osobinama. Razvoj informatike i biotehnologije rezultirao je u stvaranju bioinformatike i omogućio je širu primenu mikroarrey tehnike. Moderna biotehnologija može da dopuni i poboljša efikasnost klasičnog oplemenjivanja u cilju stvaranja visokorodnih genotipova kukuruza otpornih na bolesti i stres.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Biotechnology in maize breeding, Biotehnologija u oplemenjivanju kukuruza",
volume = "36",
number = "2",
pages = "93-109",
doi = "10.2298/GENSR0402093M"
}
Mladenović-Drinić, S., Ignjatović-Micić, D., Erić, I., Anđelković, V., Jelovac, D.,& Konstantinov, K. (2004). Biotehnologija u oplemenjivanju kukuruza.
GenetikaDruštvo genetičara Srbije, Beograd., 36(2), 93-109.
https://doi.org/10.2298/GENSR0402093M
Mladenović-Drinić S, Ignjatović-Micić D, Erić I, Anđelković V, Jelovac D, Konstantinov K. Biotehnologija u oplemenjivanju kukuruza. Genetika. 2004;36(2):93-109
Mladenović-Drinić Snežana, Ignjatović-Micić Dragana, Erić Iva, Anđelković Violeta, Jelovac Dražen, Konstantinov Kosana, "Biotehnologija u oplemenjivanju kukuruza" 36, no. 2 (2004):93-109,
https://doi.org/10.2298/GENSR0402093M .
2

Seed proteins as genetic markers in the study of phylogenetic relations in pepper

Zečević, B.; Stevanović, D.; Mladenović-Drinić, Snežana; Konstantinov, Kosana; Jakovljević, T.

(2002)

TY  - CONF
AU  - Zečević, B.
AU  - Stevanović, D.
AU  - Mladenović-Drinić, Snežana
AU  - Konstantinov, Kosana
AU  - Jakovljević, T.
PY  - 2002
UR  - http://rik.mrizp.rs/handle/123456789/37
AB  - On the basis SDS - PAGE electrophoresis of eight genotypes, which belong to different varieties of pepper (Capsicum annuum L.), was found that the seed protein profile could be useful in the study of phylogenetic relationships. The analysis of soluble seed proteins showed that all studied genotypes had a specific protein pattern. Based on obtained electrophorograms the indices of similarity were calculated. The dendrogram was constructed using an unweighted pair - group method with arithmetical averages (UPGMA) and computer program NTSYS - pe. The distinctness of genotypes, within different varieties and also within the same variety, has been confirmed. The clustering of investigated genotypes on dendrogram is not complete according to the classifications within Capsicum annuum L., which were done on the basis morphological traits. The highest values of the index of similarity was calculated for the genotypes KP068 (C. annum var. chipca) and KP075 (C. annuum var. chipca), while the lowest values were obtained for the genotypes KP005 (C. annuum var. grossum) and KP049 (C. annuum var. longum). Considering the importance of proper systematisation for successful work on pepper breeding, the results of this investigation could be useful as an attempt to clarify taxonomy, origin and phylogenetic affinities within Capsicum annuum L.
C3  - Acta Horticulturae
T1  - Seed proteins as genetic markers in the study of phylogenetic relations in pepper
VL  - 579
SP  - 113
EP  - 116
DO  - 10.17660/ActaHortic.2002.579.15
ER  - 
@conference{
author = "Zečević, B. and Stevanović, D. and Mladenović-Drinić, Snežana and Konstantinov, Kosana and Jakovljević, T.",
year = "2002",
url = "http://rik.mrizp.rs/handle/123456789/37",
abstract = "On the basis SDS - PAGE electrophoresis of eight genotypes, which belong to different varieties of pepper (Capsicum annuum L.), was found that the seed protein profile could be useful in the study of phylogenetic relationships. The analysis of soluble seed proteins showed that all studied genotypes had a specific protein pattern. Based on obtained electrophorograms the indices of similarity were calculated. The dendrogram was constructed using an unweighted pair - group method with arithmetical averages (UPGMA) and computer program NTSYS - pe. The distinctness of genotypes, within different varieties and also within the same variety, has been confirmed. The clustering of investigated genotypes on dendrogram is not complete according to the classifications within Capsicum annuum L., which were done on the basis morphological traits. The highest values of the index of similarity was calculated for the genotypes KP068 (C. annum var. chipca) and KP075 (C. annuum var. chipca), while the lowest values were obtained for the genotypes KP005 (C. annuum var. grossum) and KP049 (C. annuum var. longum). Considering the importance of proper systematisation for successful work on pepper breeding, the results of this investigation could be useful as an attempt to clarify taxonomy, origin and phylogenetic affinities within Capsicum annuum L.",
journal = "Acta Horticulturae",
title = "Seed proteins as genetic markers in the study of phylogenetic relations in pepper",
volume = "579",
pages = "113-116",
doi = "10.17660/ActaHortic.2002.579.15"
}
Zečević, B., Stevanović, D., Mladenović-Drinić, S., Konstantinov, K.,& Jakovljević, T. (2002). Seed proteins as genetic markers in the study of phylogenetic relations in pepper.
Acta Horticulturae, 579, 113-116.
https://doi.org/10.17660/ActaHortic.2002.579.15
Zečević B, Stevanović D, Mladenović-Drinić S, Konstantinov K, Jakovljević T. Seed proteins as genetic markers in the study of phylogenetic relations in pepper. Acta Horticulturae. 2002;579:113-116
Zečević B., Stevanović D., Mladenović-Drinić Snežana, Konstantinov Kosana, Jakovljević T., "Seed proteins as genetic markers in the study of phylogenetic relations in pepper" 579 (2002):113-116,
https://doi.org/10.17660/ActaHortic.2002.579.15 .
2
5

Genetically modified plant-benefits and risk

Mladenović-Drinić, Snežana; Konstantinov, Kosana; Drinić, Goran

(Institut PKB Agroekonomik, Padinska skela, 2002)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Konstantinov, Kosana
AU  - Drinić, Goran
PY  - 2002
UR  - http://rik.mrizp.rs/handle/123456789/41
AB  - Modern biotechnology is applied in agriculture, together with classical technology, in aim to increase production and improve quality of food. Revolutionary event for the plant biotechnology was possibility of creations of genetically modified plants, in fact, possibility of introducing of the genes from any organisms, to the plant genome. A genetic modified plant has the potential to offer very significant improvements in the quantity quality and acceptability of the world's food supply. First genetically modified plant were generated less than 18 years ago and now many GM plants are beginning to widely grown and products of these plants are available in the market place. Since the first commercial introduction of a genetically modified plant growing area has increase rapidly to more than 50 mill ha. The first generation of genetically modified crops with "input "traits as insect resistance, herbicide tolerance, delayed ripening, virus resistance are currently on the market.
AB  - Moderna biotehnologija se primenjuje u poljoprivredi, zajedno sa klasičnim tehnologijama, u cilju povećanja proizvodnje hrane i poboljšanja njenog kvaliteta. Revolucioni pomak na polju biotehnologije biljaka predstavlja mogućnost stvaranja genetički modifikovanih biljaka tj. mogućnost ugrađivanja gena poreklom iz bilo kog organizma u genom biljke. Genetički modifikovane biljke imaju mogućnost da obezbede više, bolje i bezbednije proizvode koji zajedno sa klasičnom tehnologijom, povećavaju proizvodnju hrane koja će moći da zadovolji potrebe rastuće ljudske populacije. Prva genetički modifikovana biljka je dobijena pre osamnaest godina, a danas se gaje komercijalno mnoge genetički modifikovane biljke i proizvodi od njih su dostupni na tržištu. Prva generacija genetički modifikovanih biljaka za takozvane ²input² osobine obuhvata tolerantnost na totalne herbicide rezistentnost na insekte, rezistentnost na viruse. Od prve komercijalizacije genetički modifikovanih biljaka površine na kojima se gaje su značajno povećane na više od 50 mil ha.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Genetically modified plant-benefits and risk
T1  - Genetički modifikovane biljke - koristi i rizici
VL  - 8
IS  - 1
SP  - 17
EP  - 24
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Konstantinov, Kosana and Drinić, Goran",
year = "2002",
url = "http://rik.mrizp.rs/handle/123456789/41",
abstract = "Modern biotechnology is applied in agriculture, together with classical technology, in aim to increase production and improve quality of food. Revolutionary event for the plant biotechnology was possibility of creations of genetically modified plants, in fact, possibility of introducing of the genes from any organisms, to the plant genome. A genetic modified plant has the potential to offer very significant improvements in the quantity quality and acceptability of the world's food supply. First genetically modified plant were generated less than 18 years ago and now many GM plants are beginning to widely grown and products of these plants are available in the market place. Since the first commercial introduction of a genetically modified plant growing area has increase rapidly to more than 50 mill ha. The first generation of genetically modified crops with "input "traits as insect resistance, herbicide tolerance, delayed ripening, virus resistance are currently on the market., Moderna biotehnologija se primenjuje u poljoprivredi, zajedno sa klasičnim tehnologijama, u cilju povećanja proizvodnje hrane i poboljšanja njenog kvaliteta. Revolucioni pomak na polju biotehnologije biljaka predstavlja mogućnost stvaranja genetički modifikovanih biljaka tj. mogućnost ugrađivanja gena poreklom iz bilo kog organizma u genom biljke. Genetički modifikovane biljke imaju mogućnost da obezbede više, bolje i bezbednije proizvode koji zajedno sa klasičnom tehnologijom, povećavaju proizvodnju hrane koja će moći da zadovolji potrebe rastuće ljudske populacije. Prva genetički modifikovana biljka je dobijena pre osamnaest godina, a danas se gaje komercijalno mnoge genetički modifikovane biljke i proizvodi od njih su dostupni na tržištu. Prva generacija genetički modifikovanih biljaka za takozvane ²input² osobine obuhvata tolerantnost na totalne herbicide rezistentnost na insekte, rezistentnost na viruse. Od prve komercijalizacije genetički modifikovanih biljaka površine na kojima se gaje su značajno povećane na više od 50 mil ha.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Genetically modified plant-benefits and risk, Genetički modifikovane biljke - koristi i rizici",
volume = "8",
number = "1",
pages = "17-24"
}
Mladenović-Drinić, S., Konstantinov, K.,& Drinić, G. (2002). Genetički modifikovane biljke - koristi i rizici.
Zbornik naučnih radova Instituta PKB AgroekonomikInstitut PKB Agroekonomik, Padinska skela., 8(1), 17-24.
Mladenović-Drinić S, Konstantinov K, Drinić G. Genetički modifikovane biljke - koristi i rizici. Zbornik naučnih radova Instituta PKB Agroekonomik. 2002;8(1):17-24
Mladenović-Drinić Snežana, Konstantinov Kosana, Drinić Goran, "Genetički modifikovane biljke - koristi i rizici" 8, no. 1 (2002):17-24

Biotechnology: Reality or dream

Konstantinov, Kosana; Mladenović-Drinić, Snežana; Drinić, Goran

(Društvo genetičara Srbije, Beograd, 2002)

TY  - JOUR
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
AU  - Drinić, Goran
PY  - 2002
UR  - http://rik.mrizp.rs/handle/123456789/30
AB  - The development of molecular biology and molecular genetics, especially of the recombinant DNA technology enabled improvement of experimental methods that provide manipulation within a cell-free system, such as cell and tissue cultures. Such methods resulted in the development of different new technologies with specific properties in relation to the conventional definitions. According to PERSLEY and lantin (2000) the following components are essential for the contemporary biotechnology: (i) genomics - a molecular characterization of all genes and gene products of an organism (ii) bioinformatics - the assembly of data from genomic analysis into accessible forms; (iii) transformation - the introduction of genes controlling a trait of interest into a genome of a desired organism (micro organisms, plants, animal systems). By the application of cotemporary biotechnology new methods in the field of diagnostic are developed such as rapid and more accurate identification of the presence and absence of genes in the genome of the organism of interest (identification of pathogens prenatal diagnostics, molecular markers assisted breeding for plants, etc). The traits of an organism are determined by its genetic material, i.e. by a molecule of deoxyribonucleic acid (DNA). watson and crick (1953) were the first scientists to describe the structure of DNA as a double-stranded helix. Higher organisms contain a set of linear DNA molecules - chromosomes and a full set of chromosomes of an organism is a genome. Each genome is divided into a series of functional units, i.e. genes. The traits of an organism depend on genes, but their expression depends not only on genes but also on many other factors, including whether a gene, controlling the trait, expresses, specific cells in which it expresses and specially the mode by which the gene and its product interact with the environment. A special aspect within the application of biotechnology occurs as an interaction of a foreign gene with a genome of an integrated organism. Also application of biotechnology provides transfer of one or several favorable genes from any evolutionary category into other category of an organism and in such a way it is possible to develop genetically modified organisms (GMO) having expressed desired, target traits. A survey of the application of biotechnology in the world and our country is presented in this paper.
AB  - Razvoj molekularne biologije i molekularne genetike posebno tehnologije rekombinantne DNK uz istovremeno usavršavanje tehnologija koje omogućavaju manipulacije u bezćelijskom sistemu kao što su kultura ćelija i tkiva rezultirao je u formiranju oblasti biotehnologije sa specifičnim osobinama u odnosu na klasične definicije. Integracijom najpropulzivnijih naučnih disciplina kao što su biologija, biohemija, molekularna biologija, genetika hemijski inženjering i informatika otvorene su neograničene mogućnosti i izazovi u sprečavanju bolesti humane populacije, biljaka i životinja kontroli insekata bez pesticida, povećanju produktivnosti stoke, povećanju proizvodnje i poboljšanju kvaliteta hrane i redukciji životne sredine. Osnovne komponente moderne biotehnologije, prema Presley-u i Lantin (2000) su: (i) genomika - molekularna karakterizacija svih gena i produkata gena organizma; (ii) bioinformatika - organizovanje podataka dobijenih iz analize genoma u prihvatljivu formu; (iii) transformacija - unošenje gena koji kontroliše osobinu od interesa u genom željenog organizma (mikroorganizmi biljke, animalni sistemi) i (iv) dijagnostika - brža i pouzdanija identifikacija prisustva ili odsustva gena u genomu organizma od interesa (identifikacija patogena, prenatalna dijagnostika, molekularno oplemenjivanje biljaka korišćenjem molekularnih markera i sl). Svojstva organizma su određena njegovim genetičkim materijalom, odnosno molekulom dezoksiribonukleinske kiseline (DNK). Watson i Crick (1953) su prvi objasnili strukturu molekula DNK kao dvolančane zavojnice. Viši organizmi sadrže set linearnih DNK molekula - hromozoma i kompletan set hromozoma organizma je genom. Svaki genom je podeljen u serije funkcionalnih jedinica odnosno gena. Svojstva organizma zavise od gena ali njihovo ispoljavanje zavisi i od mnogih drugih faktora, uključujući da li se gen odgovoran za tu osobinu eksprimira, specifičnih ćelija u kojima se eksprimira a posebno kako gen i njegov produkt intereaguju sa spoljašnjom sredinom. Poseban aspekt u okviru korišćenja biotehnologije se javlja kao interakcija stranog gena sa genomom organizma u koji je integrisan. Primena tehnologije rekombinatne DNK ima nekoliko prednosti u odnosu na tradicionalno oplemenjivanje. Genetičke modifikacije biljaka kao i klasično oplemenjivanje imaju isti cilj, dobijanje biljaka sa poboljšanim svojstvima. Klasičnim metodama oplemenjivanja prenosi se kompletan set gena iz roditeljskih linija u novo potomstvo. Time se ne prenosi samo jedan ili dva željena gena već stotine drugih gena, neki poželjni, a neki ne. Takođe, tradicionalne metode oplemenjivanja se zasnivaju na prenosu genetičkog materijala između jedinki iste ili veoma bliske vrste. Primenom biotehnologije moguće je preneti jedan ili više poželjnih gena iz bilo koje evolucione kategorije u istu ili drugu kategoriju organizma i na taj način stvoriti genetički modifikovane organizme (GMO) sa željenim osobinama. U radu će biti dat pregled stanja u oblasti primene biotehnologije u svetu i kod nas.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Biotechnology: Reality or dream
T1  - Biotehnologija - stvarnost ili san
VL  - 34
IS  - 2-3
SP  - 101
EP  - 113
DO  - 10.2298/GENSR0203101K
ER  - 
@article{
author = "Konstantinov, Kosana and Mladenović-Drinić, Snežana and Drinić, Goran",
year = "2002",
url = "http://rik.mrizp.rs/handle/123456789/30",
abstract = "The development of molecular biology and molecular genetics, especially of the recombinant DNA technology enabled improvement of experimental methods that provide manipulation within a cell-free system, such as cell and tissue cultures. Such methods resulted in the development of different new technologies with specific properties in relation to the conventional definitions. According to PERSLEY and lantin (2000) the following components are essential for the contemporary biotechnology: (i) genomics - a molecular characterization of all genes and gene products of an organism (ii) bioinformatics - the assembly of data from genomic analysis into accessible forms; (iii) transformation - the introduction of genes controlling a trait of interest into a genome of a desired organism (micro organisms, plants, animal systems). By the application of cotemporary biotechnology new methods in the field of diagnostic are developed such as rapid and more accurate identification of the presence and absence of genes in the genome of the organism of interest (identification of pathogens prenatal diagnostics, molecular markers assisted breeding for plants, etc). The traits of an organism are determined by its genetic material, i.e. by a molecule of deoxyribonucleic acid (DNA). watson and crick (1953) were the first scientists to describe the structure of DNA as a double-stranded helix. Higher organisms contain a set of linear DNA molecules - chromosomes and a full set of chromosomes of an organism is a genome. Each genome is divided into a series of functional units, i.e. genes. The traits of an organism depend on genes, but their expression depends not only on genes but also on many other factors, including whether a gene, controlling the trait, expresses, specific cells in which it expresses and specially the mode by which the gene and its product interact with the environment. A special aspect within the application of biotechnology occurs as an interaction of a foreign gene with a genome of an integrated organism. Also application of biotechnology provides transfer of one or several favorable genes from any evolutionary category into other category of an organism and in such a way it is possible to develop genetically modified organisms (GMO) having expressed desired, target traits. A survey of the application of biotechnology in the world and our country is presented in this paper., Razvoj molekularne biologije i molekularne genetike posebno tehnologije rekombinantne DNK uz istovremeno usavršavanje tehnologija koje omogućavaju manipulacije u bezćelijskom sistemu kao što su kultura ćelija i tkiva rezultirao je u formiranju oblasti biotehnologije sa specifičnim osobinama u odnosu na klasične definicije. Integracijom najpropulzivnijih naučnih disciplina kao što su biologija, biohemija, molekularna biologija, genetika hemijski inženjering i informatika otvorene su neograničene mogućnosti i izazovi u sprečavanju bolesti humane populacije, biljaka i životinja kontroli insekata bez pesticida, povećanju produktivnosti stoke, povećanju proizvodnje i poboljšanju kvaliteta hrane i redukciji životne sredine. Osnovne komponente moderne biotehnologije, prema Presley-u i Lantin (2000) su: (i) genomika - molekularna karakterizacija svih gena i produkata gena organizma; (ii) bioinformatika - organizovanje podataka dobijenih iz analize genoma u prihvatljivu formu; (iii) transformacija - unošenje gena koji kontroliše osobinu od interesa u genom željenog organizma (mikroorganizmi biljke, animalni sistemi) i (iv) dijagnostika - brža i pouzdanija identifikacija prisustva ili odsustva gena u genomu organizma od interesa (identifikacija patogena, prenatalna dijagnostika, molekularno oplemenjivanje biljaka korišćenjem molekularnih markera i sl). Svojstva organizma su određena njegovim genetičkim materijalom, odnosno molekulom dezoksiribonukleinske kiseline (DNK). Watson i Crick (1953) su prvi objasnili strukturu molekula DNK kao dvolančane zavojnice. Viši organizmi sadrže set linearnih DNK molekula - hromozoma i kompletan set hromozoma organizma je genom. Svaki genom je podeljen u serije funkcionalnih jedinica odnosno gena. Svojstva organizma zavise od gena ali njihovo ispoljavanje zavisi i od mnogih drugih faktora, uključujući da li se gen odgovoran za tu osobinu eksprimira, specifičnih ćelija u kojima se eksprimira a posebno kako gen i njegov produkt intereaguju sa spoljašnjom sredinom. Poseban aspekt u okviru korišćenja biotehnologije se javlja kao interakcija stranog gena sa genomom organizma u koji je integrisan. Primena tehnologije rekombinatne DNK ima nekoliko prednosti u odnosu na tradicionalno oplemenjivanje. Genetičke modifikacije biljaka kao i klasično oplemenjivanje imaju isti cilj, dobijanje biljaka sa poboljšanim svojstvima. Klasičnim metodama oplemenjivanja prenosi se kompletan set gena iz roditeljskih linija u novo potomstvo. Time se ne prenosi samo jedan ili dva željena gena već stotine drugih gena, neki poželjni, a neki ne. Takođe, tradicionalne metode oplemenjivanja se zasnivaju na prenosu genetičkog materijala između jedinki iste ili veoma bliske vrste. Primenom biotehnologije moguće je preneti jedan ili više poželjnih gena iz bilo koje evolucione kategorije u istu ili drugu kategoriju organizma i na taj način stvoriti genetički modifikovane organizme (GMO) sa željenim osobinama. U radu će biti dat pregled stanja u oblasti primene biotehnologije u svetu i kod nas.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Biotechnology: Reality or dream, Biotehnologija - stvarnost ili san",
volume = "34",
number = "2-3",
pages = "101-113",
doi = "10.2298/GENSR0203101K"
}
Konstantinov, K., Mladenović-Drinić, S.,& Drinić, G. (2002). Biotehnologija - stvarnost ili san.
GenetikaDruštvo genetičara Srbije, Beograd., 34(2-3), 101-113.
https://doi.org/10.2298/GENSR0203101K
Konstantinov K, Mladenović-Drinić S, Drinić G. Biotehnologija - stvarnost ili san. Genetika. 2002;34(2-3):101-113
Konstantinov Kosana, Mladenović-Drinić Snežana, Drinić Goran, "Biotehnologija - stvarnost ili san" 34, no. 2-3 (2002):101-113,
https://doi.org/10.2298/GENSR0203101K .
1

Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers

Mladenović-Drinić, Snežana; Trifunović, Slobodan; Drinić, Goran; Konstantinov, Kosana

(2002)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Trifunović, Slobodan
AU  - Drinić, Goran
AU  - Konstantinov, Kosana
PY  - 2002
UR  - http://rik.mrizp.rs/handle/123456789/38
AB  - Simple Sequence Repeats molecular markers have been used to analyse the genetic relationship among twelve maize inbred lines and to predict heterosis in their crosses. Genetic distance for 66 crosses among 12 inbred lines ranged from 0,123 between pairs Mo17 and ZPL70/9 up to 0.064 between B84 and L155. The UPGMA clustering grouped the inbreds into three clusters. The cluster I consist of inbred lines derived from BSSS germplasm or germplasm related to it. The cluster II contains the Lancaster lines, while cluster III includs two independent lines. Date shows that inbreds most closely related by their pedigree are also closely related on the basis of marker information. The correlation coefficient between heterosis for grain yield and genetic distances based on SSR markers are positive and mainly significant, while their magnitude is not large enough to be beneficiary in prediction of heterosis.
T2  - Maydica
T1  - Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers
VL  - 47
IS  - 1
SP  - 1
EP  - 8
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Trifunović, Slobodan and Drinić, Goran and Konstantinov, Kosana",
year = "2002",
url = "http://rik.mrizp.rs/handle/123456789/38",
abstract = "Simple Sequence Repeats molecular markers have been used to analyse the genetic relationship among twelve maize inbred lines and to predict heterosis in their crosses. Genetic distance for 66 crosses among 12 inbred lines ranged from 0,123 between pairs Mo17 and ZPL70/9 up to 0.064 between B84 and L155. The UPGMA clustering grouped the inbreds into three clusters. The cluster I consist of inbred lines derived from BSSS germplasm or germplasm related to it. The cluster II contains the Lancaster lines, while cluster III includs two independent lines. Date shows that inbreds most closely related by their pedigree are also closely related on the basis of marker information. The correlation coefficient between heterosis for grain yield and genetic distances based on SSR markers are positive and mainly significant, while their magnitude is not large enough to be beneficiary in prediction of heterosis.",
journal = "Maydica",
title = "Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers",
volume = "47",
number = "1",
pages = "1-8"
}
Mladenović-Drinić, S., Trifunović, S., Drinić, G.,& Konstantinov, K. (2002). Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers.
Maydica, 47(1), 1-8.
Mladenović-Drinić S, Trifunović S, Drinić G, Konstantinov K. Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers. Maydica. 2002;47(1):1-8
Mladenović-Drinić Snežana, Trifunović Slobodan, Drinić Goran, Konstantinov Kosana, "Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers" 47, no. 1 (2002):1-8
27

Genetically characterization and simple purity checking of maize seed by protein markers

Mladenović-Drinić, Snežana; Konstantinov, Kosana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2001)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Konstantinov, Kosana
PY  - 2001
UR  - http://rik.mrizp.rs/handle/123456789/26
AB  - Purity control and genetically characterization of maize inbred lines and hybrids have great importance in every phase of experimental or commercial hybrid seed production and inbred maintenance. Embryo proteins as genetic markers for maize genotypic identification as well as genetic purity determination were used. By electrophoresis embryo proteins have been separated into numerous components and showed great heterogeneity. All analyzed genotypes have specific protein pattern. Some of protein fraction appeared for each genotype at the same position and could be use as reference bands. The differences of protein complex among various maize variet­ies could be use to detect relationship between hybrids and their parental lines. The method is rapid, cheap and date can be use to form genetically identification card of inbred lines and hybrids.
AB  - Kontrola genetičke čistoće i identifikacija genotipa je od velikog značaja u svim fazama proizvodnje eksperimentalnih i komercijalnih hibrida i održavanju linija kukuruza. U radu su korišćeni proteini klice za genetičku karakterizaciju genotipova kukuruza kao i za utvrđivanje genetičke čistoće semena. Elektroforezom proteini klice su razdvojeni na veliki broj frakcija koje pokazuju visoku heterogenost. Svi ispitani genotipovi kukuruza imali su jedinstvenu proteinsku sliku pri čemu se određen broj proteinskih frakcija nalazi na približno istoj poziciji u svim ispitanim uzorcima tako da se koriste kao referentne. Razlike u proteinskom sastavu linija mogu da se koriste i za utvrđivanje veze između hibrida i roditeljskih linija. Metod je brz, jeftin i podaci mogu da se koriste za formiranje genetičke identifikacione karte linija i hibrida kukuruza.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Genetically characterization and simple purity checking of maize seed by protein markers
T1  - Korišćenje proteinskih markera za karakterizaciju i utvrđivanje genetičke čistoće semena kukuruza
VL  - 8
IS  - 1-4
SP  - 45
EP  - 49
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Konstantinov, Kosana",
year = "2001",
url = "http://rik.mrizp.rs/handle/123456789/26",
abstract = "Purity control and genetically characterization of maize inbred lines and hybrids have great importance in every phase of experimental or commercial hybrid seed production and inbred maintenance. Embryo proteins as genetic markers for maize genotypic identification as well as genetic purity determination were used. By electrophoresis embryo proteins have been separated into numerous components and showed great heterogeneity. All analyzed genotypes have specific protein pattern. Some of protein fraction appeared for each genotype at the same position and could be use as reference bands. The differences of protein complex among various maize variet­ies could be use to detect relationship between hybrids and their parental lines. The method is rapid, cheap and date can be use to form genetically identification card of inbred lines and hybrids., Kontrola genetičke čistoće i identifikacija genotipa je od velikog značaja u svim fazama proizvodnje eksperimentalnih i komercijalnih hibrida i održavanju linija kukuruza. U radu su korišćeni proteini klice za genetičku karakterizaciju genotipova kukuruza kao i za utvrđivanje genetičke čistoće semena. Elektroforezom proteini klice su razdvojeni na veliki broj frakcija koje pokazuju visoku heterogenost. Svi ispitani genotipovi kukuruza imali su jedinstvenu proteinsku sliku pri čemu se određen broj proteinskih frakcija nalazi na približno istoj poziciji u svim ispitanim uzorcima tako da se koriste kao referentne. Razlike u proteinskom sastavu linija mogu da se koriste i za utvrđivanje veze između hibrida i roditeljskih linija. Metod je brz, jeftin i podaci mogu da se koriste za formiranje genetičke identifikacione karte linija i hibrida kukuruza.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Genetically characterization and simple purity checking of maize seed by protein markers, Korišćenje proteinskih markera za karakterizaciju i utvrđivanje genetičke čistoće semena kukuruza",
volume = "8",
number = "1-4",
pages = "45-49"
}
Mladenović-Drinić, S.,& Konstantinov, K. (2001). Korišćenje proteinskih markera za karakterizaciju i utvrđivanje genetičke čistoće semena kukuruza.
Selekcija i semenarstvoDruštvo selekcionara i semenara Republike Srbije, Beograd., 8(1-4), 45-49.
Mladenović-Drinić S, Konstantinov K. Korišćenje proteinskih markera za karakterizaciju i utvrđivanje genetičke čistoće semena kukuruza. Selekcija i semenarstvo. 2001;8(1-4):45-49
Mladenović-Drinić Snežana, Konstantinov Kosana, "Korišćenje proteinskih markera za karakterizaciju i utvrđivanje genetičke čistoće semena kukuruza" 8, no. 1-4 (2001):45-49

Application of molecular markers and bioinformatics in plant breeding

Mladenović-Drinić, Snežana; Konstantinov, Kosana; Drinić, Goran; Jelovac, Dražen

(Društvo genetičara Srbije, Beograd, 2000)

TY  - JOUR
AU  - Mladenović-Drinić, Snežana
AU  - Konstantinov, Kosana
AU  - Drinić, Goran
AU  - Jelovac, Dražen
PY  - 2000
UR  - http://rik.mrizp.rs/handle/123456789/15
AB  - In the past decade development of molecular genetics brought new dimension of a plant breeding. Molecular markers as universally methods for all biological system virtually effect on a success of directly examination of structure and function of genome and well as determination of genetic polymorphism of plant genomes. The potential applications of molecular markers in plant breeding are: analysis of molecular basis of evolution; germ plasma identification classification and management; assessing genetic diversity; identification of genes underlying agronomy important traits as yield, resistance to stress and disease as well as heterosis. High density genetic linkage maps for a number of plant species as a basis for marker assisted selection of agronomically useful traits and isolation of these genes have been established. Important factor in the successes of the genetic improvement of crops was the development of faster and more reliable methods, which allowed easier analysis of date as well as rapid information exchange. The application of information technology and development of statistical techniques to analyze genomic information is know as 'bioinformatics'. By integrating genetics with informatics investigations of whole genomes aims to elucidate the structure function and evolution of plant genomes are faster. Together these technologies as integral part of classical breeding programs contributing significantly to shorting of plant breeding process and cycles of selection.
AB  - Tokom poslednje decenije razvoj molekularne biologije uneo je novu dimenziju u oplemenjivanje biljaka. Molekularni markeri kao univerzalna metoda za sve sisteme je značajno uticala na uspeh direktnog ispitivanja strukture i funkcije genoma kao i ispitivanje genetičkog polimorfizma. Potencijalne primene molekularnih markera u oplemenjivanju biljaka su: ispitivanje molekularne osnove evolucije; identifikacija, klasifikacija i održavanje germplazme; ispitivanje genetičke raznovrsnosti; identifikacija gena koji kontrolišu prinos, heterosis, stres ili bolesti. Formirane su visoko zgusnute genetičke mape za brojne biljne vrste kao osnova selekcije zasnovane na markerima za agronomski važne osobine kao i izolovanje gena. Važan faktor u uspehu genetičkog poboljšanja biljaka je razvoj brzih i pouzdanih metoda koji omogućavaju lakšu analizu podataka kao i brzu razmenu informacija. Primena informatike i razvoj statističkih metoda za analizu genetičkih informacija poznata je kao 'bioinformatika'. Integracijom genetike sa informatikom ispitivanja na nivou genoma sa ciljem da se rasvetli struktura, funkcija i evolucija biljnog genoma su ubrzana. Zajedno ove tehnologije kao sastavni deo konvencionalnih programa oplemenjivanja značajno doprinose skraćenju procesa selekcije.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Application of molecular markers and bioinformatics in plant breeding
T1  - Primena molekularnih markera i bioinformatike u oplemenjivanju biljaka
VL  - 32
IS  - 2
SP  - 93
EP  - 113
ER  - 
@article{
author = "Mladenović-Drinić, Snežana and Konstantinov, Kosana and Drinić, Goran and Jelovac, Dražen",
year = "2000",
url = "http://rik.mrizp.rs/handle/123456789/15",
abstract = "In the past decade development of molecular genetics brought new dimension of a plant breeding. Molecular markers as universally methods for all biological system virtually effect on a success of directly examination of structure and function of genome and well as determination of genetic polymorphism of plant genomes. The potential applications of molecular markers in plant breeding are: analysis of molecular basis of evolution; germ plasma identification classification and management; assessing genetic diversity; identification of genes underlying agronomy important traits as yield, resistance to stress and disease as well as heterosis. High density genetic linkage maps for a number of plant species as a basis for marker assisted selection of agronomically useful traits and isolation of these genes have been established. Important factor in the successes of the genetic improvement of crops was the development of faster and more reliable methods, which allowed easier analysis of date as well as rapid information exchange. The application of information technology and development of statistical techniques to analyze genomic information is know as 'bioinformatics'. By integrating genetics with informatics investigations of whole genomes aims to elucidate the structure function and evolution of plant genomes are faster. Together these technologies as integral part of classical breeding programs contributing significantly to shorting of plant breeding process and cycles of selection., Tokom poslednje decenije razvoj molekularne biologije uneo je novu dimenziju u oplemenjivanje biljaka. Molekularni markeri kao univerzalna metoda za sve sisteme je značajno uticala na uspeh direktnog ispitivanja strukture i funkcije genoma kao i ispitivanje genetičkog polimorfizma. Potencijalne primene molekularnih markera u oplemenjivanju biljaka su: ispitivanje molekularne osnove evolucije; identifikacija, klasifikacija i održavanje germplazme; ispitivanje genetičke raznovrsnosti; identifikacija gena koji kontrolišu prinos, heterosis, stres ili bolesti. Formirane su visoko zgusnute genetičke mape za brojne biljne vrste kao osnova selekcije zasnovane na markerima za agronomski važne osobine kao i izolovanje gena. Važan faktor u uspehu genetičkog poboljšanja biljaka je razvoj brzih i pouzdanih metoda koji omogućavaju lakšu analizu podataka kao i brzu razmenu informacija. Primena informatike i razvoj statističkih metoda za analizu genetičkih informacija poznata je kao 'bioinformatika'. Integracijom genetike sa informatikom ispitivanja na nivou genoma sa ciljem da se rasvetli struktura, funkcija i evolucija biljnog genoma su ubrzana. Zajedno ove tehnologije kao sastavni deo konvencionalnih programa oplemenjivanja značajno doprinose skraćenju procesa selekcije.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Application of molecular markers and bioinformatics in plant breeding, Primena molekularnih markera i bioinformatike u oplemenjivanju biljaka",
volume = "32",
number = "2",
pages = "93-113"
}
Mladenović-Drinić, S., Konstantinov, K., Drinić, G.,& Jelovac, D. (2000). Primena molekularnih markera i bioinformatike u oplemenjivanju biljaka.
GenetikaDruštvo genetičara Srbije, Beograd., 32(2), 93-113.
Mladenović-Drinić S, Konstantinov K, Drinić G, Jelovac D. Primena molekularnih markera i bioinformatike u oplemenjivanju biljaka. Genetika. 2000;32(2):93-113
Mladenović-Drinić Snežana, Konstantinov Kosana, Drinić Goran, Jelovac Dražen, "Primena molekularnih markera i bioinformatike u oplemenjivanju biljaka" 32, no. 2 (2000):93-113

Polymorphism of protein markers in Serbian spruce half-sib lines

Šijačić-Nikolić, Mirjana; Isajev, Vasilije; Konstantinov, Kosana; Mladenović-Drinić, Snežana

(Univerzitet u Beogradu - Šumarski fakultet, Beograd, 2000)

TY  - JOUR
AU  - Šijačić-Nikolić, Mirjana
AU  - Isajev, Vasilije
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
PY  - 2000
UR  - http://rik.mrizp.rs/handle/123456789/9
AB  - This paper present the results of determination of the degree of genetic polymorphism of Serbian spruce half-sib lines belonging to different varieties: var .'semidihotomy', var.'serbica' and var.'nana', incorporated in the seedling seed orchard, by the application of protein markers. The analysis in the salts of soluble proteins of the endosperms of 12 Serbian spruce genotypes was performed by separation - electrophoresis on the polyacrylamide gel using Leammli (1970) method. The obtained electrophoregrams were the base for the calculation of the coefficient of similarity, by Sheen (1972) formula, both between individuals within the lines and between the lines. The genetic distance of the analyzed material was assessed by cluster analysis. The study results show that phenotypic variability between the analyzed varieties of Serbian spruce is genetically determined.
AB  - U radu su prikazani rezultati utvrđivanja stepena genetskog polimorfizma linija polusrodnika, ugrađenih u generativnu semensku plantažu omorike koji pripadaju različitim varijetetima omorike: var. 'semidihotomy', var.'serbica' i var. 'nana', primenom proteinskih markera. Analiza u solima rastvorljivih proteina, semena 12 genotipova omorike, obavljena je razdvajanjem-elektroforezom na poliakrilamidnom gelu metodom Leammli (1970). Dobijeni elektroforegrami poslužili su kao osnova za izračunavanje koeficijenta sličnosti po formuli Sheen-a (1972), kako između individua unutar samih linija, tako i među linijma. Genetska bliskost odnosno udaljenost između analiziranog materijala utvrđena je primenom Klaster analize.
PB  - Univerzitet u Beogradu - Šumarski fakultet, Beograd
T2  - Glasnik Šumarskog fakulteta
T1  - Polymorphism of protein markers in Serbian spruce half-sib lines
T1  - Polimorfizam proteinskih markera kod half-sib linija omorike
IS  - 83
SP  - 179
EP  - 184
ER  - 
@article{
author = "Šijačić-Nikolić, Mirjana and Isajev, Vasilije and Konstantinov, Kosana and Mladenović-Drinić, Snežana",
year = "2000",
url = "http://rik.mrizp.rs/handle/123456789/9",
abstract = "This paper present the results of determination of the degree of genetic polymorphism of Serbian spruce half-sib lines belonging to different varieties: var .'semidihotomy', var.'serbica' and var.'nana', incorporated in the seedling seed orchard, by the application of protein markers. The analysis in the salts of soluble proteins of the endosperms of 12 Serbian spruce genotypes was performed by separation - electrophoresis on the polyacrylamide gel using Leammli (1970) method. The obtained electrophoregrams were the base for the calculation of the coefficient of similarity, by Sheen (1972) formula, both between individuals within the lines and between the lines. The genetic distance of the analyzed material was assessed by cluster analysis. The study results show that phenotypic variability between the analyzed varieties of Serbian spruce is genetically determined., U radu su prikazani rezultati utvrđivanja stepena genetskog polimorfizma linija polusrodnika, ugrađenih u generativnu semensku plantažu omorike koji pripadaju različitim varijetetima omorike: var. 'semidihotomy', var.'serbica' i var. 'nana', primenom proteinskih markera. Analiza u solima rastvorljivih proteina, semena 12 genotipova omorike, obavljena je razdvajanjem-elektroforezom na poliakrilamidnom gelu metodom Leammli (1970). Dobijeni elektroforegrami poslužili su kao osnova za izračunavanje koeficijenta sličnosti po formuli Sheen-a (1972), kako između individua unutar samih linija, tako i među linijma. Genetska bliskost odnosno udaljenost između analiziranog materijala utvrđena je primenom Klaster analize.",
publisher = "Univerzitet u Beogradu - Šumarski fakultet, Beograd",
journal = "Glasnik Šumarskog fakulteta",
title = "Polymorphism of protein markers in Serbian spruce half-sib lines, Polimorfizam proteinskih markera kod half-sib linija omorike",
number = "83",
pages = "179-184"
}
Šijačić-Nikolić, M., Isajev, V., Konstantinov, K.,& Mladenović-Drinić, S. (2000). Polimorfizam proteinskih markera kod half-sib linija omorike.
Glasnik Šumarskog fakultetaUniverzitet u Beogradu - Šumarski fakultet, Beograd.(83), 179-184.
Šijačić-Nikolić M, Isajev V, Konstantinov K, Mladenović-Drinić S. Polimorfizam proteinskih markera kod half-sib linija omorike. Glasnik Šumarskog fakulteta. 2000;(83):179-184
Šijačić-Nikolić Mirjana, Isajev Vasilije, Konstantinov Kosana, Mladenović-Drinić Snežana, "Polimorfizam proteinskih markera kod half-sib linija omorike", no. 83 (2000):179-184

Reliable level of maize genome polymorphism: Genome structure or expression

Konstantinov, Kosana; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2000)

TY  - JOUR
AU  - Konstantinov, Kosana
AU  - Mladenović-Drinić, Snežana
PY  - 2000
UR  - http://rik.mrizp.rs/handle/123456789/10
AB  - As biological functions in differentiated cells, except for cell division are expressed at the chromatin level, it is very important to collect more information on the actual level of polymorphism in order to understand biological processes better, including the genetic background of the heterotic effect in different genome combinations. Maize is paleotetraploid and many genes may be present in two or more copies, may be redundant or may have evolved divergent functions. Methylation could have an important role in this process and in this study methylation site polymorphism of the maize genome has been investigated. Within the scope of the heterosis phenomena investigation, two experimental approaches have been followed: (i) existing genome interaction by analysis of total soluble protein complex in parental inbreeds and its hybrid combination different times after pollination and (ii) the use of maize transgenic plants, obtained by NPT II marker gene integration. Transgenic hybrids expressed changes in izoenzyme pattern induced by bacterial gene integration in original inbred line. The bacterial gene also induced heritable shortening of the period of plant development before flowering.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Reliable level of maize genome polymorphism: Genome structure or expression
VL  - 32
IS  - 3
SP  - 431
EP  - 446
ER  - 
@article{
author = "Konstantinov, Kosana and Mladenović-Drinić, Snežana",
year = "2000",
url = "http://rik.mrizp.rs/handle/123456789/10",
abstract = "As biological functions in differentiated cells, except for cell division are expressed at the chromatin level, it is very important to collect more information on the actual level of polymorphism in order to understand biological processes better, including the genetic background of the heterotic effect in different genome combinations. Maize is paleotetraploid and many genes may be present in two or more copies, may be redundant or may have evolved divergent functions. Methylation could have an important role in this process and in this study methylation site polymorphism of the maize genome has been investigated. Within the scope of the heterosis phenomena investigation, two experimental approaches have been followed: (i) existing genome interaction by analysis of total soluble protein complex in parental inbreeds and its hybrid combination different times after pollination and (ii) the use of maize transgenic plants, obtained by NPT II marker gene integration. Transgenic hybrids expressed changes in izoenzyme pattern induced by bacterial gene integration in original inbred line. The bacterial gene also induced heritable shortening of the period of plant development before flowering.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Reliable level of maize genome polymorphism: Genome structure or expression",
volume = "32",
number = "3",
pages = "431-446"
}
Konstantinov, K.,& Mladenović-Drinić, S. (2000). Reliable level of maize genome polymorphism: Genome structure or expression.
GenetikaDruštvo genetičara Srbije, Beograd., 32(3), 431-446.
Konstantinov K, Mladenović-Drinić S. Reliable level of maize genome polymorphism: Genome structure or expression. Genetika. 2000;32(3):431-446
Konstantinov Kosana, Mladenović-Drinić Snežana, "Reliable level of maize genome polymorphism: Genome structure or expression" 32, no. 3 (2000):431-446