Sort By
Publication Year
Deposit Date
Title
Type
Access
Publication Year
2017 (1)
Type
Version
M-Rank
M23 (1)
Access

Stepanović, Jelena

Link to this page

Authority KeyName Variants
f3de5781-7190-470c-aeea-f9501feabc36
  • Stepanović, Jelena (1)
Projects

Author's Bibliography

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/682",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia.
GenetikaDruštvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. Genetika. 2017;49(1):355-364
Obradović Ana, Stanković Slavica, Nikolić Ana, Ignjatović-Micić Dragana, Krnjaja Vesna, Stepanović Jelena, Duduk Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .
3
3
3