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Kovinčić, Anika

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orcid::0000-0001-8217-161X
  • Kovinčić, Anika (6)
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Dynamics of phosphorus accumulation in maize inbred lines grain

Kovinčić, Anika; Dragičević, Vesna; Marković, Ksenija; Srdić, Jelena; Kravić, Natalija

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2016)

TY  - JOUR
AU  - Kovinčić, Anika
AU  - Dragičević, Vesna
AU  - Marković, Ksenija
AU  - Srdić, Jelena
AU  - Kravić, Natalija
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/635
AB  - Mineral elements deficiency in food can cause serious health problems. Being one of the three most importatnt macroelements for plant nutrition, phosphorus is involved in several key processes: photosynthesis, respiration, synthesis of starch, transport of carbohydrates and products of photosynthesis, cell division and increased water utilization. The objective of this study was to estimate the dynamics of inorganic phosphorus (Pi), as a precursor in the synthesis of phytic acid, and phytic phosphorus (Pphy) accumulation during the grain filling of two maize inbred lines differing in FAO maturity groups - L217 and L773. In addition, possible linkage between phosphorus content in grain and agro-morphological performances was observed. For the analysis of the dynamics in Pi and Pphy contents, seed samples were taken at 15th, 30th, 45th and 60th day after the polination. The obtained results showed a continuous decline of Pi content in both genotypes, with the most intensive decline observed at the first interval (e.i. 15-30 days) after the polination. The trend observed was much more pronounced in L217. Moreover, this inbred acheaved higher grain yield for 61.8 % at first sowing and for 63.1 % at 10-day delayed sowing, respectively, compared to inbred L773. In all three intervals observed, the decrease of Pi content was highly correlated with Pphy content increase for both genotypes. Based on lower accumulation rate, followed by lower Pphy content (2.635 mg g-1) during the stage of physiological maturuty, it could be concluded that inbred L217 could be considered as potentialy suitable genotype for low-phytic hybride selection.
AB  - Nedostatak mineralnih elemenata u hrani, može izazvati ozbiljne zdravstvene probleme. Kao jedan od tri najvažnija makroelementa u ishrani biljaka, fosfor učestvuje u nizu procesa u biljci: fotosintezi, disanju, sintezi skroba, transportu ugljenih hidrata i produkata fotosinteze, deobi ćelija i boljem korišćenju vode. Ciljevi ovog istraživanja su uključivali praćenje dinamike nakupljanja neorganskog fosfora (Pi) - prekursora u sintezi fitinske kiseline, i fitinskog fosfora (Pphy), tokom nalivanja zrna dve samooplodne linije kukuruza različitih FAO grupa zrenja - L217 i L773, i utvrđivanje povezanosti sadržaja fosfora u zrnu i agro-morfoloških performansi. Za analizu dinamike sadržaja neorganskog i fitinskog fosfora, uzorci zrna su uzimani: 15-tog, 30- tog, 45-tog i 60-tog dana nakon oplodnje. Na osnovu dobijenih rezultata, uočen je kontinuirani pad sadržaja neorganskog fosfora kod oba genotipa, a najintezivniji pad utvrđen je u prvom intervalu (tj. 15-30 dana) nakon oplodnje, bivajući znatno izraženiji kod linije L217. Takođe, u odnosu na liniju L773, linija L217 je ostvarila viši prinos za 61,8 % u prvom, odnosno za 63,1 % u drugom setvenom roku, respektivno. Srazmerno smanjenju neorganskog fosfora, uočen je trend povećanja fitinskog fosfora kod oba genotipa tokom sva tri intervala merenja. Smanjena dinamika nakupljanja i niži sadržaj fitinskog fosfora (2,635 mg g-1) u fazi fiziološke zrelosti, ukazuje da bi se inbred linija L217 mogla smatrati potencijalno poželjnim genotipom za selekciju niskofitinskih hibrida.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Dynamics of phosphorus accumulation in maize inbred lines grain
T1  - Praćenje dinamike nakupljanja fosfora u zrnu samooplodnih linija kukuruza
VL  - 22
IS  - 2
SP  - 69
EP  - 78
DO  - 10.5937/selsem1602069K
ER  - 
@article{
author = "Kovinčić, Anika and Dragičević, Vesna and Marković, Ksenija and Srdić, Jelena and Kravić, Natalija",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/635",
abstract = "Mineral elements deficiency in food can cause serious health problems. Being one of the three most importatnt macroelements for plant nutrition, phosphorus is involved in several key processes: photosynthesis, respiration, synthesis of starch, transport of carbohydrates and products of photosynthesis, cell division and increased water utilization. The objective of this study was to estimate the dynamics of inorganic phosphorus (Pi), as a precursor in the synthesis of phytic acid, and phytic phosphorus (Pphy) accumulation during the grain filling of two maize inbred lines differing in FAO maturity groups - L217 and L773. In addition, possible linkage between phosphorus content in grain and agro-morphological performances was observed. For the analysis of the dynamics in Pi and Pphy contents, seed samples were taken at 15th, 30th, 45th and 60th day after the polination. The obtained results showed a continuous decline of Pi content in both genotypes, with the most intensive decline observed at the first interval (e.i. 15-30 days) after the polination. The trend observed was much more pronounced in L217. Moreover, this inbred acheaved higher grain yield for 61.8 % at first sowing and for 63.1 % at 10-day delayed sowing, respectively, compared to inbred L773. In all three intervals observed, the decrease of Pi content was highly correlated with Pphy content increase for both genotypes. Based on lower accumulation rate, followed by lower Pphy content (2.635 mg g-1) during the stage of physiological maturuty, it could be concluded that inbred L217 could be considered as potentialy suitable genotype for low-phytic hybride selection., Nedostatak mineralnih elemenata u hrani, može izazvati ozbiljne zdravstvene probleme. Kao jedan od tri najvažnija makroelementa u ishrani biljaka, fosfor učestvuje u nizu procesa u biljci: fotosintezi, disanju, sintezi skroba, transportu ugljenih hidrata i produkata fotosinteze, deobi ćelija i boljem korišćenju vode. Ciljevi ovog istraživanja su uključivali praćenje dinamike nakupljanja neorganskog fosfora (Pi) - prekursora u sintezi fitinske kiseline, i fitinskog fosfora (Pphy), tokom nalivanja zrna dve samooplodne linije kukuruza različitih FAO grupa zrenja - L217 i L773, i utvrđivanje povezanosti sadržaja fosfora u zrnu i agro-morfoloških performansi. Za analizu dinamike sadržaja neorganskog i fitinskog fosfora, uzorci zrna su uzimani: 15-tog, 30- tog, 45-tog i 60-tog dana nakon oplodnje. Na osnovu dobijenih rezultata, uočen je kontinuirani pad sadržaja neorganskog fosfora kod oba genotipa, a najintezivniji pad utvrđen je u prvom intervalu (tj. 15-30 dana) nakon oplodnje, bivajući znatno izraženiji kod linije L217. Takođe, u odnosu na liniju L773, linija L217 je ostvarila viši prinos za 61,8 % u prvom, odnosno za 63,1 % u drugom setvenom roku, respektivno. Srazmerno smanjenju neorganskog fosfora, uočen je trend povećanja fitinskog fosfora kod oba genotipa tokom sva tri intervala merenja. Smanjena dinamika nakupljanja i niži sadržaj fitinskog fosfora (2,635 mg g-1) u fazi fiziološke zrelosti, ukazuje da bi se inbred linija L217 mogla smatrati potencijalno poželjnim genotipom za selekciju niskofitinskih hibrida.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Dynamics of phosphorus accumulation in maize inbred lines grain, Praćenje dinamike nakupljanja fosfora u zrnu samooplodnih linija kukuruza",
volume = "22",
number = "2",
pages = "69-78",
doi = "10.5937/selsem1602069K"
}
Kovinčić, A., Dragičević, V., Marković, K., Srdić, J.,& Kravić, N. (2016). Praćenje dinamike nakupljanja fosfora u zrnu samooplodnih linija kukuruza.
Selekcija i semenarstvoDruštvo selekcionara i semenara Republike Srbije, Beograd., 22(2), 69-78.
https://doi.org/10.5937/selsem1602069K
Kovinčić A, Dragičević V, Marković K, Srdić J, Kravić N. Praćenje dinamike nakupljanja fosfora u zrnu samooplodnih linija kukuruza. Selekcija i semenarstvo. 2016;22(2):69-78
Kovinčić Anika, Dragičević Vesna, Marković Ksenija, Srdić Jelena, Kravić Natalija, "Praćenje dinamike nakupljanja fosfora u zrnu samooplodnih linija kukuruza" 22, no. 2 (2016):69-78,
https://doi.org/10.5937/selsem1602069K .

Study of the susceptibility of maize lines to some sulfonylurea herbicides

Dragičević, Vesna; Simić, Milena; Sečanski, Mile; Cvijanović, Gorica; Kovinčić, Anika

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Simić, Milena
AU  - Sečanski, Mile
AU  - Cvijanović, Gorica
AU  - Kovinčić, Anika
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/469
AB  - Maize lines are susceptible to different herbicides, what makes seed production more complicate. The susceptibility is depending in high extent on meteorological conditions. The objective of the study was to investigate genetic variability and correlation between phytotoxicity (EWRC evaluation), alterations in dry matter (DM), phenolics and soluble proteins (SP) in sets of 19 ZP lines, to nicosulfuron and foramsulfuron, with the aim to determine sensitivity of individual lines and the potential tolerance patterns during period 2006-2009. The negative impact of both herbicides reflects through DM increase, but in higher degree in nicosulfuron treatment. They also induced in average SP increase and decrease of phenolic’s content. According to different meteorological conditions present during examined four years, SP increase was followed by increased EWRC values at same lines, opposite to lines in which decrease of SP and increase of phenolic’s level could be tied to potential tolerance, what gives importance to testing of each individual maize line. Special attention must be given to application time (according to meteorological conditions and level of weed infestation). The decrease in SP level and increase of phenolics, together with lower EWRC values observed at some lines could be associated to tolerance patterns what was emphasized particularly during 2009, when visible injuries were absent.
AB  - Linije kukuruza su osetljive prema različitim herbicidima, što otežava semensku proizvodnju. Osetljivost zavisi u velikoj meri od meteoroloških uslova. Objekat ispitivanja je utvrđivanje genetičke varijabilnosti i korelacije između fitotoksičnosti (EWRC ocena) i promena suve materije (DM), fenola i rastvorljivih proteina (SP) kod 19 ZP linija prema nikosulfuronu i foramsulfuronu, sa ciljem da se utvrdi osetljivost pojedinačnih linija, kao i model potencijalne tolerantnosti, tokom perioda 2006-2009. Negativan uticaj oba herbicida se odrazio ne povećanje DM, ali u većem stepenu je na to uticao nikosulfuron. Primenjeni herbicidi su takođe uticali na povećanje prosečnog sadržaja SP i smanjenje sadržaja fenola. Zahvaljujući različitim meteorološkim uslovima prisutnim tokom četiri godine ispitivanja, povećanje SP je bilo praćeno povećanjem EWRC vrednosti kod istih linija, suprotno od linija kod kojih bi se pad SP i povećanje nivoa fenola mogli vezati za potencijalnu tolerantnost, što naglašava važnost testiranja svake pojedinačne linije. Posebna pažnja bi se trebala posvetiti vremenu upotrebe herbicida (vezano za na meteorološke uslove i zakorovljenost). Smanjenje SP i povećanje sadržaja fenola kod pojedinih linija zajedno sa nižim EWRC vrednostima bi moglo biti povezano sa njihovom tolerantnošću, što je posebno bilo naglašeno tokom 2009, kada nisu bila zabeležena vidljiva oštećenja kod biljaka.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Study of the susceptibility of maize lines to some sulfonylurea herbicides
T1  - Ispitivanje osetljivosti linija kukuruza prema nekim sulfonilurea herbicidima
VL  - 44
IS  - 2
SP  - 355
EP  - 366
DO  - 10.2298/GENSR1202355D
ER  - 
@article{
author = "Dragičević, Vesna and Simić, Milena and Sečanski, Mile and Cvijanović, Gorica and Kovinčić, Anika",
year = "2012",
url = "http://rik.mrizp.rs/handle/123456789/469",
abstract = "Maize lines are susceptible to different herbicides, what makes seed production more complicate. The susceptibility is depending in high extent on meteorological conditions. The objective of the study was to investigate genetic variability and correlation between phytotoxicity (EWRC evaluation), alterations in dry matter (DM), phenolics and soluble proteins (SP) in sets of 19 ZP lines, to nicosulfuron and foramsulfuron, with the aim to determine sensitivity of individual lines and the potential tolerance patterns during period 2006-2009. The negative impact of both herbicides reflects through DM increase, but in higher degree in nicosulfuron treatment. They also induced in average SP increase and decrease of phenolic’s content. According to different meteorological conditions present during examined four years, SP increase was followed by increased EWRC values at same lines, opposite to lines in which decrease of SP and increase of phenolic’s level could be tied to potential tolerance, what gives importance to testing of each individual maize line. Special attention must be given to application time (according to meteorological conditions and level of weed infestation). The decrease in SP level and increase of phenolics, together with lower EWRC values observed at some lines could be associated to tolerance patterns what was emphasized particularly during 2009, when visible injuries were absent., Linije kukuruza su osetljive prema različitim herbicidima, što otežava semensku proizvodnju. Osetljivost zavisi u velikoj meri od meteoroloških uslova. Objekat ispitivanja je utvrđivanje genetičke varijabilnosti i korelacije između fitotoksičnosti (EWRC ocena) i promena suve materije (DM), fenola i rastvorljivih proteina (SP) kod 19 ZP linija prema nikosulfuronu i foramsulfuronu, sa ciljem da se utvrdi osetljivost pojedinačnih linija, kao i model potencijalne tolerantnosti, tokom perioda 2006-2009. Negativan uticaj oba herbicida se odrazio ne povećanje DM, ali u većem stepenu je na to uticao nikosulfuron. Primenjeni herbicidi su takođe uticali na povećanje prosečnog sadržaja SP i smanjenje sadržaja fenola. Zahvaljujući različitim meteorološkim uslovima prisutnim tokom četiri godine ispitivanja, povećanje SP je bilo praćeno povećanjem EWRC vrednosti kod istih linija, suprotno od linija kod kojih bi se pad SP i povećanje nivoa fenola mogli vezati za potencijalnu tolerantnost, što naglašava važnost testiranja svake pojedinačne linije. Posebna pažnja bi se trebala posvetiti vremenu upotrebe herbicida (vezano za na meteorološke uslove i zakorovljenost). Smanjenje SP i povećanje sadržaja fenola kod pojedinih linija zajedno sa nižim EWRC vrednostima bi moglo biti povezano sa njihovom tolerantnošću, što je posebno bilo naglašeno tokom 2009, kada nisu bila zabeležena vidljiva oštećenja kod biljaka.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Study of the susceptibility of maize lines to some sulfonylurea herbicides, Ispitivanje osetljivosti linija kukuruza prema nekim sulfonilurea herbicidima",
volume = "44",
number = "2",
pages = "355-366",
doi = "10.2298/GENSR1202355D"
}
Dragičević, V., Simić, M., Sečanski, M., Cvijanović, G.,& Kovinčić, A. (2012). Ispitivanje osetljivosti linija kukuruza prema nekim sulfonilurea herbicidima.
GenetikaDruštvo genetičara Srbije, Beograd., 44(2), 355-366.
https://doi.org/10.2298/GENSR1202355D
Dragičević V, Simić M, Sečanski M, Cvijanović G, Kovinčić A. Ispitivanje osetljivosti linija kukuruza prema nekim sulfonilurea herbicidima. Genetika. 2012;44(2):355-366
Dragičević Vesna, Simić Milena, Sečanski Mile, Cvijanović Gorica, Kovinčić Anika, "Ispitivanje osetljivosti linija kukuruza prema nekim sulfonilurea herbicidima" 44, no. 2 (2012):355-366,
https://doi.org/10.2298/GENSR1202355D .
3
3

Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds

Dragičević, Vesna; Sredojević, Slobodanka; Perić, Vesna; Kovinčić, Anika; Srebrić, Mirjana

(Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad, 2011)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Sredojević, Slobodanka
AU  - Perić, Vesna
AU  - Kovinčić, Anika
AU  - Srebrić, Mirjana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/405
AB  - Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds.
AB  - Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
T2  - Acta periodica technologica
T1  - Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds
T1  - Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu
IS  - 42
SP  - 11
EP  - 21
DO  - 10.2298/APT1142011D
ER  - 
@article{
author = "Dragičević, Vesna and Sredojević, Slobodanka and Perić, Vesna and Kovinčić, Anika and Srebrić, Mirjana",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/405",
abstract = "Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD) H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA) were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds., Fitat je važan za razvoj semena i služi za skladištenje minerala. On se uglavnom smatra za antinutritivnu supstancu. Cilj istraživanja je bio da se razvije brza i jeftina kolorimetrijska metoda za merenje koncentracije fitata i neorganskog fosfora (Pi) iz ekstrakta semena kukuruza, soje i suncokreta, kombinujući određenu preciznost i jednostavnost, idealne za selekcionere zainte- resovane za istovremeno poboljšanje odnosa Pi i fitata u semenu. Ispitivana ekstrakciona sredstva: bidestilovana voda (DD H2O), 2,4 % HCl i 5 % trihlorsirćetna kiselina (TCA) su se pokazale pogodnim za analizu fitinske kiseline i Pi iz ekstrakta semena. Prednost 5 % TCA u odnosu na DD H2O i 2,4 % HCl se ogleda u niskoj granici detekcije za fitinsku kiselinu i Pi, dobrom recoveryju i niskim vrednostima odstupanja (bias). Niska granica detekcije za Pi je važna za uzorke sa prirodno niskom Pi koncentracijom, kao što je seme soje.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad",
journal = "Acta periodica technologica",
title = "Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds, Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu",
number = "42",
pages = "11-21",
doi = "10.2298/APT1142011D"
}
Dragičević, V., Sredojević, S., Perić, V., Kovinčić, A.,& Srebrić, M. (2011). Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu.
Acta periodica technologicaUniverzitet u Novom Sadu - Tehnološki fakultet, Novi Sad.(42), 11-21.
https://doi.org/10.2298/APT1142011D
Dragičević V, Sredojević S, Perić V, Kovinčić A, Srebrić M. Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu. Acta periodica technologica. 2011;(42):11-21
Dragičević Vesna, Sredojević Slobodanka, Perić Vesna, Kovinčić Anika, Srebrić Mirjana, "Studija validacije brze kolorimetrijske metode za određivanje fitinske kiseline i neorganskog fosfora u semenu", no. 42 (2011):11-21,
https://doi.org/10.2298/APT1142011D .
10
25

Characterization of sunflower seed and kernel proteins

Žilić, Slađana; Barać, Miroljub; Pešić, Mirjana; Crevar, Miloš; Stanojević, Slađana; Kovinčić, Anika; Saratlić, Goran; Tolimir, Miodrag

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
AU  - Crevar, Miloš
AU  - Stanojević, Slađana
AU  - Kovinčić, Anika
AU  - Saratlić, Goran
AU  - Tolimir, Miodrag
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/292
AB  - Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 - 42,900 Da and α', Mw = 31,000 - 35,300 Da), while one was basic (β, Mw=21,000 - 29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P lt 0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P lt 0.05).
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Characterization of sunflower seed and kernel proteins
VL  - 33
IS  - 52
SP  - 103
EP  - 113
DO  - 10.2298/HEL1052103Z
ER  - 
@article{
author = "Žilić, Slađana and Barać, Miroljub and Pešić, Mirjana and Crevar, Miloš and Stanojević, Slađana and Kovinčić, Anika and Saratlić, Goran and Tolimir, Miodrag",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/292",
abstract = "Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 - 42,900 Da and α', Mw = 31,000 - 35,300 Da), while one was basic (β, Mw=21,000 - 29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P lt 0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P lt 0.05).",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Characterization of sunflower seed and kernel proteins",
volume = "33",
number = "52",
pages = "103-113",
doi = "10.2298/HEL1052103Z"
}
Žilić, S., Barać, M., Pešić, M., Crevar, M., Stanojević, S., Kovinčić, A., Saratlić, G.,& Tolimir, M. (2010). Characterization of sunflower seed and kernel proteins.
HeliaInstitut za ratarstvo i povrtarstvo, Novi Sad., 33(52), 103-113.
https://doi.org/10.2298/HEL1052103Z
Žilić S, Barać M, Pešić M, Crevar M, Stanojević S, Kovinčić A, Saratlić G, Tolimir M. Characterization of sunflower seed and kernel proteins. Helia. 2010;33(52):103-113
Žilić Slađana, Barać Miroljub, Pešić Mirjana, Crevar Miloš, Stanojević Slađana, Kovinčić Anika, Saratlić Goran, Tolimir Miodrag, "Characterization of sunflower seed and kernel proteins" 33, no. 52 (2010):103-113,
https://doi.org/10.2298/HEL1052103Z .
13
17

Antioxidants in soybean and sunflower grain

Dragičević, Vesna; Perić, Vesna; Kovinčić, Anika; Srebrić, Mirjana

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Perić, Vesna
AU  - Kovinčić, Anika
AU  - Srebrić, Mirjana
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/294
AB  - The objective of this study was to examine differences in antioxidants content: phytate, phenolics, free thiolics (PSH) and glutathione (GSH) in seeds of 7 soybean varieties and 10 sunflower lines with aim to signify their nutritive quality, valuable for further breeding process. The variations between soybean varieties in phytate content were minor, while el/7 was sunflower line with lowest phytate content, which could be used for program breeding of low phytate grain. The relative high phytate and phenolics content was present in Laura seeds, as well as lowest PSH and GSH content. Generally, the higher content of phenolics were in sunflower seeds, what could be considered as negative atribut from nutritive point of view. The highest PSH content was observed in soybean seeds of Nena and Olga. Lower PSH and GSH content was noted in sunflower seeds (down to 4 and 7 times, respectively), compared to soybean. The soybean seeds have potentially better antioxidative potential, compared to sunflower, owing to multiple higher PSH and GSH content and lower level of phenolics. Soybean variety Olga is accenuated as high in PSH and GSH, as well as sunflower line l4/ru, which had higher PSH and particulary GSH level, with lower phytate and phenolics content, as possible antinutrients.
AB  - Cilj ogleda je bio da se ispitaju razlike u sadržaju antioksidanata: fitina, fenola, slobodnih tiola i glutationa u semenu 7 ZP sorti soje i 10 linija suncokreta i time ukaže na njihov nutritivni kvalitet i značaj za dalji proces selekcije. Kod soje nije bilo znatnijih variranja u pogledu sadržaja fitina, dok se kod suncokreta izdvaja el/7, kao linija sa najnižim učešćem fitina, koja bi mogla poslužiti u postupku oplemenjvanja niskofitinskih hibrida suncokreta. Kod Laure je pored relativno visokog sadržaj fenola i fitina bio prisutan i najniži sadržaj PSH i GSH. Kod suncokreta je generalno bio prisutan znatno veći sadržaj fenola u zrnu, što je sa nutritivne tačke gledišta negativno. Kod ispitivanih sorti soje, najveći sadržaj PSH je bio kod Nene i Olge. U semenu suncokreta je prosečno bilo 4 i 7 puta manje PSH i GSH. Seme soje poseduje potencijalno bolji antioksidativni potencijal u odnosu na suncokret, zahvaljujući višestruko većem sadržaju PSH i GSH i nižem udelu fenola. Posebno se ističe sorta soje Olga sa visokom sadržajem PSH i GSH, kao i linija suncokreta l4/ru, koja je imala veći sadržaj PSH, a posebno GSH i niži udeo fitina i fenola, kao potencijalnih antinutritiva.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Antioxidants in soybean and sunflower grain
T1  - Antioksidanti u semenu soje i suncokreta
VL  - 59
IS  - 3-4
SP  - 393
EP  - 399
ER  - 
@article{
author = "Dragičević, Vesna and Perić, Vesna and Kovinčić, Anika and Srebrić, Mirjana",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/294",
abstract = "The objective of this study was to examine differences in antioxidants content: phytate, phenolics, free thiolics (PSH) and glutathione (GSH) in seeds of 7 soybean varieties and 10 sunflower lines with aim to signify their nutritive quality, valuable for further breeding process. The variations between soybean varieties in phytate content were minor, while el/7 was sunflower line with lowest phytate content, which could be used for program breeding of low phytate grain. The relative high phytate and phenolics content was present in Laura seeds, as well as lowest PSH and GSH content. Generally, the higher content of phenolics were in sunflower seeds, what could be considered as negative atribut from nutritive point of view. The highest PSH content was observed in soybean seeds of Nena and Olga. Lower PSH and GSH content was noted in sunflower seeds (down to 4 and 7 times, respectively), compared to soybean. The soybean seeds have potentially better antioxidative potential, compared to sunflower, owing to multiple higher PSH and GSH content and lower level of phenolics. Soybean variety Olga is accenuated as high in PSH and GSH, as well as sunflower line l4/ru, which had higher PSH and particulary GSH level, with lower phytate and phenolics content, as possible antinutrients., Cilj ogleda je bio da se ispitaju razlike u sadržaju antioksidanata: fitina, fenola, slobodnih tiola i glutationa u semenu 7 ZP sorti soje i 10 linija suncokreta i time ukaže na njihov nutritivni kvalitet i značaj za dalji proces selekcije. Kod soje nije bilo znatnijih variranja u pogledu sadržaja fitina, dok se kod suncokreta izdvaja el/7, kao linija sa najnižim učešćem fitina, koja bi mogla poslužiti u postupku oplemenjvanja niskofitinskih hibrida suncokreta. Kod Laure je pored relativno visokog sadržaj fenola i fitina bio prisutan i najniži sadržaj PSH i GSH. Kod suncokreta je generalno bio prisutan znatno veći sadržaj fenola u zrnu, što je sa nutritivne tačke gledišta negativno. Kod ispitivanih sorti soje, najveći sadržaj PSH je bio kod Nene i Olge. U semenu suncokreta je prosečno bilo 4 i 7 puta manje PSH i GSH. Seme soje poseduje potencijalno bolji antioksidativni potencijal u odnosu na suncokret, zahvaljujući višestruko većem sadržaju PSH i GSH i nižem udelu fenola. Posebno se ističe sorta soje Olga sa visokom sadržajem PSH i GSH, kao i linija suncokreta l4/ru, koja je imala veći sadržaj PSH, a posebno GSH i niži udeo fitina i fenola, kao potencijalnih antinutritiva.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Antioxidants in soybean and sunflower grain, Antioksidanti u semenu soje i suncokreta",
volume = "59",
number = "3-4",
pages = "393-399"
}
Dragičević, V., Perić, V., Kovinčić, A.,& Srebrić, M. (2010). Antioksidanti u semenu soje i suncokreta.
Savremena poljoprivredaUniverzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 59(3-4), 393-399.
Dragičević V, Perić V, Kovinčić A, Srebrić M. Antioksidanti u semenu soje i suncokreta. Savremena poljoprivreda. 2010;59(3-4):393-399
Dragičević Vesna, Perić Vesna, Kovinčić Anika, Srebrić Mirjana, "Antioksidanti u semenu soje i suncokreta" 59, no. 3-4 (2010):393-399

The quantitative determination of seed mass defect during germination, 1: The daily dynamics of net supplemental free energy

Sredojević, Slobodanka; Dragičević, Vesna; Srebrić, Mirjana; Perić, Vesna; Kovinčić, Anika; Đukanović, Lana

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2008)

TY  - JOUR
AU  - Sredojević, Slobodanka
AU  - Dragičević, Vesna
AU  - Srebrić, Mirjana
AU  - Perić, Vesna
AU  - Kovinčić, Anika
AU  - Đukanović, Lana
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/224
AB  - A dynamics of seed mass defect and a previous process of the water input during germination was determined by a trial with five soybean cultivars and four sunflower hybrids, following the ISTA Rules. It was noticed that leaching, which led to the mass defect (smDef), was permanent during 8 and 10 days of soybean and sunflower germination, respectively. The two confronted processes, the liquid input and the solid output, could be operable at the level of a thermodynamic parameter of Gibbs free energy (G), i.e. net supplemental free energy (netGspl). Hence, this type of free energy - net supplement, differentiates in two examined plant species (between the first and the last day of germination): soybean in a range from -53.23 to -69.82 J mol-1 seedling-1 and sunflower from -46.35 to -91.94 J mol-1 seedling-1. Furthermore, the daily change of net supplemental energy underlined the three points ingenotypes of both species: after the first, before the last day of germination and in the maximum (-67.50 and -75.25 J mol-1 seedling-1 in soybean, then, -94.97 and -103.79 J mol-1 seedling-1 in sunflower) between the 4th and the 7th day of germination depending on a genotype.
AB  - U ovom radu izneta su kvantitativna određivanja dinamike gubitka mase semena tokom klijanja kod pet sorti soje i četiri hibrida suncokreta po ISTA pravilima. Kako je metoda određivanja masenog defekta semena davala i dinamiku usvajanja vode, ta dva podatka prevedena u Gibsovu slobodnu energiju omogućila su operaciju sa dva suprotstavljena procesa: usvajanja tečne i ispuštanja čvrste supstance, koje su oduzimanjem dale neto energiju unetu u sistem seme-klijanac. Pokazalo se da neto, dopunska slobodna energija u opsegu od -53.23 do -69.82 J mol-1 klijanac-1 za soju (od prvog do osmog dana) i od -46.35 do -91.94 J mol-1 klijanac-1 za suncokret (od prvog do desetog dana), daje razliku između dve ispitivane vrste. Dalje, dnevna promena neto, dodatne energije razdvaja genotipove kod obe biljne vrste u tri tačke: posle prvog dana, pre zadnjeg dana i maksimum (-67.50 i -75.25 J mol-1 klijanac-1 za soju, a -94.97 i -103.79 J mol-1 klijanac-1 za suncokret), između četvrtog i sedmog dana, što zavisi od genotipa.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - The quantitative determination of seed mass defect during germination, 1: The daily dynamics of net supplemental free energy
T1  - Kvantitativno određivanje masenog defekta semena tokom klijanja, 1 - dnevna dinamika neto dodatne slobodne energije
VL  - 69
IS  - 4
SP  - 63
EP  - 77
ER  - 
@article{
author = "Sredojević, Slobodanka and Dragičević, Vesna and Srebrić, Mirjana and Perić, Vesna and Kovinčić, Anika and Đukanović, Lana",
year = "2008",
url = "http://rik.mrizp.rs/handle/123456789/224",
abstract = "A dynamics of seed mass defect and a previous process of the water input during germination was determined by a trial with five soybean cultivars and four sunflower hybrids, following the ISTA Rules. It was noticed that leaching, which led to the mass defect (smDef), was permanent during 8 and 10 days of soybean and sunflower germination, respectively. The two confronted processes, the liquid input and the solid output, could be operable at the level of a thermodynamic parameter of Gibbs free energy (G), i.e. net supplemental free energy (netGspl). Hence, this type of free energy - net supplement, differentiates in two examined plant species (between the first and the last day of germination): soybean in a range from -53.23 to -69.82 J mol-1 seedling-1 and sunflower from -46.35 to -91.94 J mol-1 seedling-1. Furthermore, the daily change of net supplemental energy underlined the three points ingenotypes of both species: after the first, before the last day of germination and in the maximum (-67.50 and -75.25 J mol-1 seedling-1 in soybean, then, -94.97 and -103.79 J mol-1 seedling-1 in sunflower) between the 4th and the 7th day of germination depending on a genotype., U ovom radu izneta su kvantitativna određivanja dinamike gubitka mase semena tokom klijanja kod pet sorti soje i četiri hibrida suncokreta po ISTA pravilima. Kako je metoda određivanja masenog defekta semena davala i dinamiku usvajanja vode, ta dva podatka prevedena u Gibsovu slobodnu energiju omogućila su operaciju sa dva suprotstavljena procesa: usvajanja tečne i ispuštanja čvrste supstance, koje su oduzimanjem dale neto energiju unetu u sistem seme-klijanac. Pokazalo se da neto, dopunska slobodna energija u opsegu od -53.23 do -69.82 J mol-1 klijanac-1 za soju (od prvog do osmog dana) i od -46.35 do -91.94 J mol-1 klijanac-1 za suncokret (od prvog do desetog dana), daje razliku između dve ispitivane vrste. Dalje, dnevna promena neto, dodatne energije razdvaja genotipove kod obe biljne vrste u tri tačke: posle prvog dana, pre zadnjeg dana i maksimum (-67.50 i -75.25 J mol-1 klijanac-1 za soju, a -94.97 i -103.79 J mol-1 klijanac-1 za suncokret), između četvrtog i sedmog dana, što zavisi od genotipa.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "The quantitative determination of seed mass defect during germination, 1: The daily dynamics of net supplemental free energy, Kvantitativno određivanje masenog defekta semena tokom klijanja, 1 - dnevna dinamika neto dodatne slobodne energije",
volume = "69",
number = "4",
pages = "63-77"
}
Sredojević, S., Dragičević, V., Srebrić, M., Perić, V., Kovinčić, A.,& Đukanović, L. (2008). Kvantitativno određivanje masenog defekta semena tokom klijanja, 1 - dnevna dinamika neto dodatne slobodne energije.
Journal of Scientific Agricultural ResearchSavez poljoprivrednih inženjera i tehničara, Beograd., 69(4), 63-77.
Sredojević S, Dragičević V, Srebrić M, Perić V, Kovinčić A, Đukanović L. Kvantitativno određivanje masenog defekta semena tokom klijanja, 1 - dnevna dinamika neto dodatne slobodne energije. Journal of Scientific Agricultural Research. 2008;69(4):63-77
Sredojević Slobodanka, Dragičević Vesna, Srebrić Mirjana, Perić Vesna, Kovinčić Anika, Đukanović Lana, "Kvantitativno određivanje masenog defekta semena tokom klijanja, 1 - dnevna dinamika neto dodatne slobodne energije" 69, no. 4 (2008):63-77