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Mandić, Violeta

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  • Mandić, Violeta (18)
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Author's Bibliography

Harvest time effect on quantitative and qualitative parameters of forage maize

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Đorđević, Snežana; Brankov, Milan; Mićić, Nenad; Stanojković, Aleksandar

(Lahore : Pakistan Agricultural Scientists Forum, 2021)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Đorđević, Snežana
AU  - Brankov, Milan
AU  - Mićić, Nenad
AU  - Stanojković, Aleksandar
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/811
AB  - Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial (two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain filling.
PB  - Lahore : Pakistan Agricultural Scientists Forum
T2  - Journal of Animal and Plant Sciences
T1  - Harvest time effect on quantitative and qualitative parameters of forage maize
VL  - 31
IS  - 1
SP  - 103
EP  - 107
DO  - 10.36899/JAPS.2021.1.0198
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Đorđević, Snežana and Brankov, Milan and Mićić, Nenad and Stanojković, Aleksandar",
year = "2021",
url = "http://rik.mrizp.rs/handle/123456789/811",
abstract = "Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial (two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain filling.",
publisher = "Lahore : Pakistan Agricultural Scientists Forum",
journal = "Journal of Animal and Plant Sciences",
title = "Harvest time effect on quantitative and qualitative parameters of forage maize",
volume = "31",
number = "1",
pages = "103-107",
doi = "10.36899/JAPS.2021.1.0198"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Đorđević, S., Brankov, M., Mićić, N.,& Stanojković, A. (2021). Harvest time effect on quantitative and qualitative parameters of forage maize.
Journal of Animal and Plant SciencesLahore : Pakistan Agricultural Scientists Forum., 31(1), 103-107.
https://doi.org/10.36899/JAPS.2021.1.0198
Mandić V, Bijelić Z, Krnjaja V, Đorđević S, Brankov M, Mićić N, Stanojković A. Harvest time effect on quantitative and qualitative parameters of forage maize. Journal of Animal and Plant Sciences. 2021;31(1):103-107
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Đorđević Snežana, Brankov Milan, Mićić Nenad, Stanojković Aleksandar, "Harvest time effect on quantitative and qualitative parameters of forage maize" 31, no. 1 (2021):103-107,
https://doi.org/10.36899/JAPS.2021.1.0198 .

Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate

Mandić, Violeta; Đorđević, Snežana; Bijelić, Zorica; Krnjaja, Vesna; Pantelić, Vlada; Simić, Aleksandar; Dragičević, Vesna

(Basel : Multidisciplinary Digital Publishing Institute, 2020)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Pantelić, Vlada
AU  - Simić, Aleksandar
AU  - Dragičević, Vesna
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/798
AB  - The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.
PB  - Basel : Multidisciplinary Digital Publishing Institute
T2  - Agronomy
T1  - Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate
VL  - 10
IS  - 4
SP  - 535
DO  - 10.3390/agronomy10040535
ER  - 
@article{
author = "Mandić, Violeta and Đorđević, Snežana and Bijelić, Zorica and Krnjaja, Vesna and Pantelić, Vlada and Simić, Aleksandar and Dragičević, Vesna",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/798",
abstract = "The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute",
journal = "Agronomy",
title = "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate",
volume = "10",
number = "4",
pages = "535",
doi = "10.3390/agronomy10040535"
}
Mandić, V., Đorđević, S., Bijelić, Z., Krnjaja, V., Pantelić, V., Simić, A.,& Dragičević, V. (2020). Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate.
AgronomyBasel : Multidisciplinary Digital Publishing Institute., 10(4), 535.
https://doi.org/10.3390/agronomy10040535
Mandić V, Đorđević S, Bijelić Z, Krnjaja V, Pantelić V, Simić A, Dragičević V. Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. Agronomy. 2020;10(4):535
Mandić Violeta, Đorđević Snežana, Bijelić Zorica, Krnjaja Vesna, Pantelić Vlada, Simić Aleksandar, Dragičević Vesna, "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate" 10, no. 4 (2020):535,
https://doi.org/10.3390/agronomy10040535 .
1
3
2
3

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop ProtectionElsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
6
3
3

The possibilities of weed control in the intercroping system of field pea and oats

Simić, Aleksandar; Krga, Ivan; Simić, Milena; Brankov, Milan; Vučković, Savo; Bijelić, Zorica; Mandić, Violeta

(Herbološko društvo Srbije, 2018)

TY  - JOUR
AU  - Simić, Aleksandar
AU  - Krga, Ivan
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Vučković, Savo
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/699
AB  - Field pea is a high quality fodder crop of a short vegetation period. The production of field peas as a spring crop reduces the risk of yield losses due to unfavourable winter conditions, improves soil characteristics and ensures high quality fodder at the end of the spring. Intercropping with oats is often carried out due to the fact that field pea requires support and both species have similar developmental stages. The field experiment was set up at the Institute for Animal Husbandry in Zemun in 2017, in order to study the forage yield and the possibility of weed control by cultivating the mixture of field pea and oats. Serbian varieties of field pea NS Junior and oats cultivar Dunav were included in the trial, intercropped according to the 'additive model' in two ways: 100:15% and 100:30% pea : oats, when compared to monoculture (100% pea and 100% oats). In the 100:15% intercrop model of field pea and oats , the number of weed units and their fresh and dry weight were lower than in monocultures, while the higher presence of weeds in the 100:30% model was significantly affected by the high number and biomass production of weed species Ambrosia artemisiifolia. The cultivation of oats and field pea in the mixture significantly increased the yield of fresh and dry biomass, when compared to monocultures, while there was no significant difference in yield between the different methods of peas and oats (100:15% and 100:30%) intercropping. The higher share of oats at the crop establishment stage did not increase the forage yield, indicating the dominant contribution of the field pea biomass in the mixture.
AB  - Stočni grašak predstavlja kvalitetan krmni usev kratke vegetacije. Proizvodnjom stočnog graška kao jarog useva, smanjuje se rizik gubitka prinosa usled nepovoljnih zimskih uslova, poboljšavaju karakteristike zemljišta i obezbeđuje kvalitetna krma krajem proleća. Združivanje sa ovsem se često sprovodi zbog potrebe graška za potporom i sličnih fenofaza razvića obe vrste. Poljski ogled je postavljen na Institutu za stočarstvo u Zemunu 2017. godine u cilju ispitivanja prinosa krme i mogućnosti suzbijanja korova gajenjem stočnog graška sa ovsem u smeši. U ogled su bile uključene domaća sorta graška NS Junior i ovas Dunav, koji su združeni po 'aditivnom modelu' na dva načina: 100:15% i 100:30% grašak:ovas, uz poređenje sa setvom čistih useva (100% grašak i 100% ovas). U združenom usevu stočnog graška i ovsa po modelu 100:15%, broj jedinki korova i njihova sveža i suva masa su bile manje nago u pojedinačnim usevima, dok je većoj zastupljenosti korova u modelu združivanja 100:30% veoma doprinela visoka brojnost i produkcija biomase korovske vrste Ambrosia artemisiifolia. Gajenje ovsa i stočnog graška u smeši je značajno povećalo prinos sveže i suve biomase u odnosu na pojedinačno gajene useve, dok između različitog načina združivanja graška i ovsa (100:15% i 100:30%) nije bilo značajnih razlika u prinosu. Veći udeo ovsa pri zasnivanju nije povećao prinos krme, ukazujući na dominantan doprinos biomase stočnog graška u smeši.
PB  - Herbološko društvo Srbije
T2  - Acta herbologica
T1  - The possibilities of weed control in the intercroping system of field pea and oats
T1  - Mogućnost suzbijanja korova združenim gajenjem jarog stočnog graška sa ovsem
VL  - 27
IS  - 2
SP  - 109
EP  - 119
DO  - 10.5937/ActaHerb1802109S
ER  - 
@article{
author = "Simić, Aleksandar and Krga, Ivan and Simić, Milena and Brankov, Milan and Vučković, Savo and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/699",
abstract = "Field pea is a high quality fodder crop of a short vegetation period. The production of field peas as a spring crop reduces the risk of yield losses due to unfavourable winter conditions, improves soil characteristics and ensures high quality fodder at the end of the spring. Intercropping with oats is often carried out due to the fact that field pea requires support and both species have similar developmental stages. The field experiment was set up at the Institute for Animal Husbandry in Zemun in 2017, in order to study the forage yield and the possibility of weed control by cultivating the mixture of field pea and oats. Serbian varieties of field pea NS Junior and oats cultivar Dunav were included in the trial, intercropped according to the 'additive model' in two ways: 100:15% and 100:30% pea : oats, when compared to monoculture (100% pea and 100% oats). In the 100:15% intercrop model of field pea and oats , the number of weed units and their fresh and dry weight were lower than in monocultures, while the higher presence of weeds in the 100:30% model was significantly affected by the high number and biomass production of weed species Ambrosia artemisiifolia. The cultivation of oats and field pea in the mixture significantly increased the yield of fresh and dry biomass, when compared to monocultures, while there was no significant difference in yield between the different methods of peas and oats (100:15% and 100:30%) intercropping. The higher share of oats at the crop establishment stage did not increase the forage yield, indicating the dominant contribution of the field pea biomass in the mixture., Stočni grašak predstavlja kvalitetan krmni usev kratke vegetacije. Proizvodnjom stočnog graška kao jarog useva, smanjuje se rizik gubitka prinosa usled nepovoljnih zimskih uslova, poboljšavaju karakteristike zemljišta i obezbeđuje kvalitetna krma krajem proleća. Združivanje sa ovsem se često sprovodi zbog potrebe graška za potporom i sličnih fenofaza razvića obe vrste. Poljski ogled je postavljen na Institutu za stočarstvo u Zemunu 2017. godine u cilju ispitivanja prinosa krme i mogućnosti suzbijanja korova gajenjem stočnog graška sa ovsem u smeši. U ogled su bile uključene domaća sorta graška NS Junior i ovas Dunav, koji su združeni po 'aditivnom modelu' na dva načina: 100:15% i 100:30% grašak:ovas, uz poređenje sa setvom čistih useva (100% grašak i 100% ovas). U združenom usevu stočnog graška i ovsa po modelu 100:15%, broj jedinki korova i njihova sveža i suva masa su bile manje nago u pojedinačnim usevima, dok je većoj zastupljenosti korova u modelu združivanja 100:30% veoma doprinela visoka brojnost i produkcija biomase korovske vrste Ambrosia artemisiifolia. Gajenje ovsa i stočnog graška u smeši je značajno povećalo prinos sveže i suve biomase u odnosu na pojedinačno gajene useve, dok između različitog načina združivanja graška i ovsa (100:15% i 100:30%) nije bilo značajnih razlika u prinosu. Veći udeo ovsa pri zasnivanju nije povećao prinos krme, ukazujući na dominantan doprinos biomase stočnog graška u smeši.",
publisher = "Herbološko društvo Srbije",
journal = "Acta herbologica",
title = "The possibilities of weed control in the intercroping system of field pea and oats, Mogućnost suzbijanja korova združenim gajenjem jarog stočnog graška sa ovsem",
volume = "27",
number = "2",
pages = "109-119",
doi = "10.5937/ActaHerb1802109S"
}
Simić, A., Krga, I., Simić, M., Brankov, M., Vučković, S., Bijelić, Z.,& Mandić, V. (2018). Mogućnost suzbijanja korova združenim gajenjem jarog stočnog graška sa ovsem.
Acta herbologicaHerbološko društvo Srbije., 27(2), 109-119.
https://doi.org/10.5937/ActaHerb1802109S
Simić A, Krga I, Simić M, Brankov M, Vučković S, Bijelić Z, Mandić V. Mogućnost suzbijanja korova združenim gajenjem jarog stočnog graška sa ovsem. Acta herbologica. 2018;27(2):109-119
Simić Aleksandar, Krga Ivan, Simić Milena, Brankov Milan, Vučković Savo, Bijelić Zorica, Mandić Violeta, "Mogućnost suzbijanja korova združenim gajenjem jarog stočnog graška sa ovsem" 27, no. 2 (2018):109-119,
https://doi.org/10.5937/ActaHerb1802109S .
2

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
ToxinsMdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
2
1
1

Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser

Brankov, Milan; Dragičević, Vesna; Simić, Milena; Filipović, Milomir; Kresović, Mirjana M.; Mandić, Violeta

(Scibulcom Ltd, Sofia, 2017)

TY  - JOUR
AU  - Brankov, Milan
AU  - Dragičević, Vesna
AU  - Simić, Milena
AU  - Filipović, Milomir
AU  - Kresović, Mirjana M.
AU  - Mandić, Violeta
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/694
AB  - Dominance of grass weeds in maize crop occurs due to a lack of selective herbicides for their control. With sulphonylurea herbicides this problem became under control, but a problem with selectivity was developed, particularly in maize seed crop. The effect of sulphonylureas and foliar fertiliser on maize lines was evaluated by visual estimation, grain yield, as well as the alterations in the content of antioxidants: free thiolic groups, phenolics and soluble proteins in the leaves. The proteins content did not vary significantly under the influence of herbicides, compared to the control, opposite to free thiolic groups and phenolics. The differences in the content of phenolics and thiolic groups in the treatments with herbicides plus foliar fertiliser indicated that herbicide stress was more rapidly overcome. Most of the genotypes expressed significant increase of grain yield in the treatments with foliar fertiliser, compared to control and analogous treatments with herbicides.
PB  - Scibulcom Ltd, Sofia
T2  - Journal of Environmental Protection and Ecology
T1  - Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser
VL  - 18
IS  - 4
SP  - 1440
EP  - 1449
ER  - 
@article{
author = "Brankov, Milan and Dragičević, Vesna and Simić, Milena and Filipović, Milomir and Kresović, Mirjana M. and Mandić, Violeta",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/694",
abstract = "Dominance of grass weeds in maize crop occurs due to a lack of selective herbicides for their control. With sulphonylurea herbicides this problem became under control, but a problem with selectivity was developed, particularly in maize seed crop. The effect of sulphonylureas and foliar fertiliser on maize lines was evaluated by visual estimation, grain yield, as well as the alterations in the content of antioxidants: free thiolic groups, phenolics and soluble proteins in the leaves. The proteins content did not vary significantly under the influence of herbicides, compared to the control, opposite to free thiolic groups and phenolics. The differences in the content of phenolics and thiolic groups in the treatments with herbicides plus foliar fertiliser indicated that herbicide stress was more rapidly overcome. Most of the genotypes expressed significant increase of grain yield in the treatments with foliar fertiliser, compared to control and analogous treatments with herbicides.",
publisher = "Scibulcom Ltd, Sofia",
journal = "Journal of Environmental Protection and Ecology",
title = "Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser",
volume = "18",
number = "4",
pages = "1440-1449"
}
Brankov, M., Dragičević, V., Simić, M., Filipović, M., Kresović, M. M.,& Mandić, V. (2017). Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser.
Journal of Environmental Protection and EcologyScibulcom Ltd, Sofia., 18(4), 1440-1449.
Brankov M, Dragičević V, Simić M, Filipović M, Kresović MM, Mandić V. Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser. Journal of Environmental Protection and Ecology. 2017;18(4):1440-1449
Brankov Milan, Dragičević Vesna, Simić Milena, Filipović Milomir, Kresović Mirjana M., Mandić Violeta, "Diminishing herbicide stress in maize inbred lines by application of foliar fertiliser" 18, no. 4 (2017):1440-1449
4

Fungal contamination of maize grain samples with a special focus on toxigenic genera

Krnjaja, Vesna; Stanojković, Aleksandar; Stanković, Slavica; Lukić, Miloš; Bijelić, Zorica; Mandić, Violeta; Mićić, Nenad

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Mićić, Nenad
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/664
AB  - In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi.
AB  - Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of maize grain samples with a special focus on toxigenic genera
T1  - Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove
VL  - 33
IS  - 2
SP  - 233
EP  - 241
DO  - 10.2298/BAH1702233K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Stanković, Slavica and Lukić, Miloš and Bijelić, Zorica and Mandić, Violeta and Mićić, Nenad",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/664",
abstract = "In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi., Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of maize grain samples with a special focus on toxigenic genera, Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove",
volume = "33",
number = "2",
pages = "233-241",
doi = "10.2298/BAH1702233K"
}
Krnjaja, V., Stanojković, A., Stanković, S., Lukić, M., Bijelić, Z., Mandić, V.,& Mićić, N. (2017). Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 33(2), 233-241.
https://doi.org/10.2298/BAH1702233K
Krnjaja V, Stanojković A, Stanković S, Lukić M, Bijelić Z, Mandić V, Mićić N. Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove. Biotechnology in Animal Husbandry. 2017;33(2):233-241
Krnjaja Vesna, Stanojković Aleksandar, Stanković Slavica, Lukić Miloš, Bijelić Zorica, Mandić Violeta, Mićić Nenad, "Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove" 33, no. 2 (2017):233-241,
https://doi.org/10.2298/BAH1702233K .
3

The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Simić, Aleksandar; Ružić-Muslić, Dragana; Dragičević, Vesna; Petričević, Veselin

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Simić, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Dragičević, Vesna
AU  - Petričević, Veselin
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/667
AB  - Rainfall is one of the most important environmental factors influencing crop production under dry land farming conditions. In the Republic of Serbia, the soybean is produced under rainfed conditions, and therefore online monitoring of the rainfall use efficiency (RUE) is essential for efficient management of production. The research aim was to estimate the effects of amount rainfall during the growing season (RGS) and average monthly rainfall on soybean grain yield (GY) in the Vojvodina during the sixteen year period (2000-2015). Distributions of RGS were not satisfactory and negatively influenced the expression genetic yield potential of cultivars. Rainfall deficits during the growing season limited the soybean plant reproductive growth stages leading to GY loss. The coefficient of variation indicated that RGS and monthly rainfall changed moderately from year to year. Regression equations showed that GY tended to increase with the amount of rainfall. GY had strong positive relationship with RGS and rainfall in May, July and August. Since the amount and distribution of rainfall during growing season are critical determinants of GY, soybean cultivars of shorter vegetation periods should be developed and cultivated so that maximum utilization of rainfall is ensured.
AB  - Padavine su jedan od najvažnijih faktora spoljašnje sredine koji utiče na produkciju useva u uslovima suvog ratarenja. U Republici Srbiji, soja se proizvodi u uslovima prirodnog vodnog režima, pa je praćenje efikasnosti korišćenja padavina (RUE) neophodno za efikasno upravljanje proizvodnjom. Cilj istraživanja bio je da se proceni efekat količine padavina tokom vegetacionog perioda i prosečnih mesečnih padavina na prinos zrna soje u Vojvodini tokom šestnaestogodišnjeg perioda (2000-2015). Distribucija padavina tokom vegetacionog perioda nije bila zadovoljavajuća i nepovoljno je uticala na ekspresiju genetičkog potencijala rodnosti sorti. Deficit padavina tokom vegetacionog perioda soje ograničile su faze reproduktivnog razvoja i dovele do redukcije prinosa. Koeficijenti varijacije pokazuju da se količina padavina tokom vegetacionog perioda i srednja mesečna količina padavina umereno menjaju iz godine u godinu. Regresijske jednačine su pokazale da se prinos zrna povećavao sa količinom padavina. Prinos zrna je u jakoj pozitivnoj korelaciji sa količinom padavina tokom vegetacionog perioda, u maju, julu i avgustu. S obzirom da su količina i raspored padavina u toku vegetacionog perioda kritična determinanta za prinos zrna, treba razvijati i gajiti sorte soje sa kraćim vegetacionim periodom da bi se obezbedila maksimalna iskorišćenost padavina.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina
T1  - Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini
VL  - 33
IS  - 4
SP  - 475
EP  - 486
DO  - 10.2298/BAH1704475M
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Simić, Aleksandar and Ružić-Muslić, Dragana and Dragičević, Vesna and Petričević, Veselin",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/667",
abstract = "Rainfall is one of the most important environmental factors influencing crop production under dry land farming conditions. In the Republic of Serbia, the soybean is produced under rainfed conditions, and therefore online monitoring of the rainfall use efficiency (RUE) is essential for efficient management of production. The research aim was to estimate the effects of amount rainfall during the growing season (RGS) and average monthly rainfall on soybean grain yield (GY) in the Vojvodina during the sixteen year period (2000-2015). Distributions of RGS were not satisfactory and negatively influenced the expression genetic yield potential of cultivars. Rainfall deficits during the growing season limited the soybean plant reproductive growth stages leading to GY loss. The coefficient of variation indicated that RGS and monthly rainfall changed moderately from year to year. Regression equations showed that GY tended to increase with the amount of rainfall. GY had strong positive relationship with RGS and rainfall in May, July and August. Since the amount and distribution of rainfall during growing season are critical determinants of GY, soybean cultivars of shorter vegetation periods should be developed and cultivated so that maximum utilization of rainfall is ensured., Padavine su jedan od najvažnijih faktora spoljašnje sredine koji utiče na produkciju useva u uslovima suvog ratarenja. U Republici Srbiji, soja se proizvodi u uslovima prirodnog vodnog režima, pa je praćenje efikasnosti korišćenja padavina (RUE) neophodno za efikasno upravljanje proizvodnjom. Cilj istraživanja bio je da se proceni efekat količine padavina tokom vegetacionog perioda i prosečnih mesečnih padavina na prinos zrna soje u Vojvodini tokom šestnaestogodišnjeg perioda (2000-2015). Distribucija padavina tokom vegetacionog perioda nije bila zadovoljavajuća i nepovoljno je uticala na ekspresiju genetičkog potencijala rodnosti sorti. Deficit padavina tokom vegetacionog perioda soje ograničile su faze reproduktivnog razvoja i dovele do redukcije prinosa. Koeficijenti varijacije pokazuju da se količina padavina tokom vegetacionog perioda i srednja mesečna količina padavina umereno menjaju iz godine u godinu. Regresijske jednačine su pokazale da se prinos zrna povećavao sa količinom padavina. Prinos zrna je u jakoj pozitivnoj korelaciji sa količinom padavina tokom vegetacionog perioda, u maju, julu i avgustu. S obzirom da su količina i raspored padavina u toku vegetacionog perioda kritična determinanta za prinos zrna, treba razvijati i gajiti sorte soje sa kraćim vegetacionim periodom da bi se obezbedila maksimalna iskorišćenost padavina.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The rainfall use efficiency and soybean grain yield under rainfed conditions in Vojvodina, Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini",
volume = "33",
number = "4",
pages = "475-486",
doi = "10.2298/BAH1704475M"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Simić, A., Ružić-Muslić, D., Dragičević, V.,& Petričević, V. (2017). Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 33(4), 475-486.
https://doi.org/10.2298/BAH1704475M
Mandić V, Bijelić Z, Krnjaja V, Simić A, Ružić-Muslić D, Dragičević V, Petričević V. Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini. Biotechnology in Animal Husbandry. 2017;33(4):475-486
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Simić Aleksandar, Ružić-Muslić Dragana, Dragičević Vesna, Petričević Veselin, "Efikasnost korišćenja padavina i prinos zrna soje u uslovima prirodnog vodnog režima u Vojvodini" 33, no. 4 (2017):475-486,
https://doi.org/10.2298/BAH1704475M .
5

Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest

Bijelić, Zorica; Krnjaja, Vesna; Muslic-Ruzić, Dragana; Mandić, Violeta; Škrbić, Zdenka; Lukić, Miloš; Stanković, Slavica

(Ars Docendi, Bucharest, 2017)

TY  - JOUR
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Muslic-Ruzić, Dragana
AU  - Mandić, Violeta
AU  - Škrbić, Zdenka
AU  - Lukić, Miloš
AU  - Stanković, Slavica
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/688
AB  - The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest
VL  - 22
IS  - 5
SP  - 12907
EP  - 12914
ER  - 
@article{
author = "Bijelić, Zorica and Krnjaja, Vesna and Muslic-Ruzić, Dragana and Mandić, Violeta and Škrbić, Zdenka and Lukić, Miloš and Stanković, Slavica",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/688",
abstract = "The aim of this study was to determine the presence of fungi and concentrations of mycotoxins, zearalenone, fumonisin and deoxynivalenol, in lucerne and grass-legume silages, under the influence of different doses of N fertilization and harvesting at different stages of phenological development of the plants. Studies included pure lucerne crop and mixtures of lucerne with cocksfoot, tall fescue and sainfoin, sown in different ratios, fertilized with 0, 70, 140 or 210 kgN ha(-1) and harvested in the butonization phase or at 50% flowering of lucerne plants. Results showed that the total fungi count in the silage depended on the three investigated factors. The highest total fungi count was determined in the mixture silages of lucerne, cocksfoot and tall fescue, which was well treated with different nitrogen quantities, harvested and prepared in later stages of plant development. The most commonly-occurring fungi were Fusarium species (85.5% of fungi were this genus). Of the studied mycotoxins, only DON depended on fertilization, as it reduced the concentration of DON from 0.15 to 0.07 mg kg(-1). Preventing appearance of fungi and their mycotoxins in forage and silage should begin in the field as well as during the process of preparation of silage.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest",
volume = "22",
number = "5",
pages = "12907-12914"
}
Bijelić, Z., Krnjaja, V., Muslic-Ruzić, D., Mandić, V., Škrbić, Z., Lukić, M.,& Stanković, S. (2017). Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest.
Romanian Biotechnological LettersArs Docendi, Bucharest., 22(5), 12907-12914.
Bijelić Z, Krnjaja V, Muslic-Ruzić D, Mandić V, Škrbić Z, Lukić M, Stanković S. Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest. Romanian Biotechnological Letters. 2017;22(5):12907-12914
Bijelić Zorica, Krnjaja Vesna, Muslic-Ruzić Dragana, Mandić Violeta, Škrbić Zdenka, Lukić Miloš, Stanković Slavica, "Occurrence of moulds and mycotoxins in grass-legume silages influenced by nitrogen fertilization and phenological phase at harvest" 22, no. 5 (2017):12907-12914
1

Genetic variability of free energy in a function of drought tolerance in common bean accessions

Jecmenica, Maja; Kravić, Natalija; Dragičević, Vesna; Vasić, Mirjana; Zivanović, Tomislav; Mandić, Violeta; Damnjanović, Jelena

(Društvo genetičara Srbije, Beograd, 2016)

TY  - JOUR
AU  - Jecmenica, Maja
AU  - Kravić, Natalija
AU  - Dragičević, Vesna
AU  - Vasić, Mirjana
AU  - Zivanović, Tomislav
AU  - Mandić, Violeta
AU  - Damnjanović, Jelena
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/626
AB  - Characterisation of bean genotypes, particularly local landraces is important for ongoing breeding programs, especially for drought tolerance. Susceptibility to drought is emphasized when bean is grown as a stubble crop and sown at the middle of summer. The aim of this study was to compare variability of ten bean genotypes to optimal (25 degrees C) and higher (30 degrees C) temperatures in combination with optimal (80%) and reduced (40%) field water capacity (FWC), from the point of growth (root and shoot length and fresh matter accumulation) and thermodynamic parameters of free energy (calculated parameter after drying at 60 degrees C, 105 degrees C and 130 degrees C) during the early seedlings stage. Significant and positive correlation between root length and fresh matter with free energy at symplast and chemically bound water occurred under temperature stress (i.e. 30 degrees C). Root growth and elongation were affected by drought stress, i.e. under a combination of high temperature (30 degrees C) and water deficit (40% FWC). Based on higher energy consumption, the ability of shoot to continue a growth in stressful conditions could be possible to achieve, as was confirm through significant and positive correlation between evaluated growth parameters in shoot and free energy of free water. It could be concluded that most of the examined dry bean accessions expressed some sensitivity to stress applied. Among genotypes tested, local landrace zecak expressed lesser susceptibility to stresses applied. Accession Maksa was more tolerant to changes at cytoplasmic level, while Medijana and Sataja 425 expressed root i.e. shoot stress tolerance. Those accessions could be considered as potentially drought tolerant genotypes.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic variability of free energy in a function of drought tolerance in common bean accessions
VL  - 48
IS  - 3
SP  - 1003
EP  - 1015
DO  - 10.2298/GENSR1603003J
ER  - 
@article{
author = "Jecmenica, Maja and Kravić, Natalija and Dragičević, Vesna and Vasić, Mirjana and Zivanović, Tomislav and Mandić, Violeta and Damnjanović, Jelena",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/626",
abstract = "Characterisation of bean genotypes, particularly local landraces is important for ongoing breeding programs, especially for drought tolerance. Susceptibility to drought is emphasized when bean is grown as a stubble crop and sown at the middle of summer. The aim of this study was to compare variability of ten bean genotypes to optimal (25 degrees C) and higher (30 degrees C) temperatures in combination with optimal (80%) and reduced (40%) field water capacity (FWC), from the point of growth (root and shoot length and fresh matter accumulation) and thermodynamic parameters of free energy (calculated parameter after drying at 60 degrees C, 105 degrees C and 130 degrees C) during the early seedlings stage. Significant and positive correlation between root length and fresh matter with free energy at symplast and chemically bound water occurred under temperature stress (i.e. 30 degrees C). Root growth and elongation were affected by drought stress, i.e. under a combination of high temperature (30 degrees C) and water deficit (40% FWC). Based on higher energy consumption, the ability of shoot to continue a growth in stressful conditions could be possible to achieve, as was confirm through significant and positive correlation between evaluated growth parameters in shoot and free energy of free water. It could be concluded that most of the examined dry bean accessions expressed some sensitivity to stress applied. Among genotypes tested, local landrace zecak expressed lesser susceptibility to stresses applied. Accession Maksa was more tolerant to changes at cytoplasmic level, while Medijana and Sataja 425 expressed root i.e. shoot stress tolerance. Those accessions could be considered as potentially drought tolerant genotypes.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic variability of free energy in a function of drought tolerance in common bean accessions",
volume = "48",
number = "3",
pages = "1003-1015",
doi = "10.2298/GENSR1603003J"
}
Jecmenica, M., Kravić, N., Dragičević, V., Vasić, M., Zivanović, T., Mandić, V.,& Damnjanović, J. (2016). Genetic variability of free energy in a function of drought tolerance in common bean accessions.
GenetikaDruštvo genetičara Srbije, Beograd., 48(3), 1003-1015.
https://doi.org/10.2298/GENSR1603003J
Jecmenica M, Kravić N, Dragičević V, Vasić M, Zivanović T, Mandić V, Damnjanović J. Genetic variability of free energy in a function of drought tolerance in common bean accessions. Genetika. 2016;48(3):1003-1015
Jecmenica Maja, Kravić Natalija, Dragičević Vesna, Vasić Mirjana, Zivanović Tomislav, Mandić Violeta, Damnjanović Jelena, "Genetic variability of free energy in a function of drought tolerance in common bean accessions" 48, no. 3 (2016):1003-1015,
https://doi.org/10.2298/GENSR1603003J .

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Tomić, Z.; Đorđević, S.; Stanojević, D.; Dragičević, Vesna

(Scibulcom Ltd, Sofia, 2016)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Đorđević, S.
AU  - Stanojević, D.
AU  - Dragičević, Vesna
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/616
AB  - The aim of this study was to determine the effects of the inoculation of maize seeds with mixtures of bacteria (Azotobacter chroococum, Azotobacter vinelandi, Bacillus megaterium, Bacillus licheniformis) during different phenophases (6-7 leaves, silking and wax ripeness stage) and on the nitrogen dynamics, total number of microorganisms, number of azotobacter and aminoheterotrophs in Chernozem and grain yield of maize hybrid ZP 684 during 2006, 2007 and 2008. Nitrogen amount in soil was significantly higher in 2006, due to favourable meteorological conditions for microbial activity (higher total number of microorganisms and number of azotobacter), than in 2007 and 2008. The minimum amount of nitrogen was in the stage of wax ripeness although the total number of microorganisms, azotobacter and aminoheterotrophs were the largest. Seed inoculation of maize significantly increases the values of all studied parameters.
PB  - Scibulcom Ltd, Sofia
T2  - Journal of Environmental Protection and Ecology
T1  - Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield
VL  - 17
IS  - 3
SP  - 1003
EP  - 1010
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Tomić, Z. and Đorđević, S. and Stanojević, D. and Dragičević, Vesna",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/616",
abstract = "The aim of this study was to determine the effects of the inoculation of maize seeds with mixtures of bacteria (Azotobacter chroococum, Azotobacter vinelandi, Bacillus megaterium, Bacillus licheniformis) during different phenophases (6-7 leaves, silking and wax ripeness stage) and on the nitrogen dynamics, total number of microorganisms, number of azotobacter and aminoheterotrophs in Chernozem and grain yield of maize hybrid ZP 684 during 2006, 2007 and 2008. Nitrogen amount in soil was significantly higher in 2006, due to favourable meteorological conditions for microbial activity (higher total number of microorganisms and number of azotobacter), than in 2007 and 2008. The minimum amount of nitrogen was in the stage of wax ripeness although the total number of microorganisms, azotobacter and aminoheterotrophs were the largest. Seed inoculation of maize significantly increases the values of all studied parameters.",
publisher = "Scibulcom Ltd, Sofia",
journal = "Journal of Environmental Protection and Ecology",
title = "Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield",
volume = "17",
number = "3",
pages = "1003-1010"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Tomić, Z., Đorđević, S., Stanojević, D.,& Dragičević, V. (2016). Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield.
Journal of Environmental Protection and EcologyScibulcom Ltd, Sofia., 17(3), 1003-1010.
Mandić V, Bijelić Z, Krnjaja V, Tomić Z, Đorđević S, Stanojević D, Dragičević V. Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield. Journal of Environmental Protection and Ecology. 2016;17(3):1003-1010
Mandić Violeta, Bijelić Zorica, Krnjaja Vesna, Tomić Z., Đorđević S., Stanojević D., Dragičević Vesna, "Effect of bacterial seed inoculation on nitrogen dynamics, number of bacteria in soil under maize, and maize yield" 17, no. 3 (2016):1003-1010
2

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop ProtectionElsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
12
8
10

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/566",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A. (2014). Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima. Biotechnology in Animal Husbandry. 2014;30(1):167-173
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Mandić Violeta, Tomić Z., Obradović Ana, "Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima" 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .
1

Moulds and mycotoxins in stored maize grains

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Tomić, Z.; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Tomić, Z.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/485
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Tomić, Z. and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/485",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Tomić, Z., Mandić, V.,& Bijelić, Z. (2013). Plesni i mikotoksini u uskladištenom kukuruzu.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K
Krnjaja V, Lević J, Stanković S, Petrović T, Tomić Z, Mandić V, Bijelić Z. Plesni i mikotoksini u uskladištenom kukuruzu. Biotechnology in Animal Husbandry. 2013;29(3):527-536
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Tomić Z., Mandić Violeta, Bijelić Zorica, "Plesni i mikotoksini u uskladištenom kukuruzu" 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K .
8

Occurrence of Fusarium species in maize grains for silage

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Bijelić, Zorica; Mandić, Violeta; Stojanović, Lj.

(Institut za stočarstvo, Beograd, 2011)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Stojanović, Lj.
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/385
AB  - Grain samples of two maize hybrids, medium early (ZP434) and late maturity (ZP704), collected during harvest in 2008 were investigated for contamination by fungi. Grains were plated on agar media and grown fungi were identified by morphological macroscopic and microscopic characteristics on potato-dextrose agar (PDA) and synthetic nutrient agar (SNA). Species of the genus Fusarium were the most common in both hybrids, and their presence amounted to 33.89% (ZP434) and 42% (ZP704). Other fungi of genera, Acremonium, Alternaria, Aspergillus, Chaetomium, Cladosporium, Nigrospora, Penicillium, Rhizopus and Trichotecium, were isolated from 0 to 41%. Four species belonging to the genus Fusarium were identified, of which the species F. verticillioides was the most common with 28.63% in ZP434 and 30.5% in ZP704 hybrids. The presence of F. graminearum, F. proliferatum and F. subglutinans ranged from 3% (ZP704) to 5% (ZP434), 0.13% (ZP434) to 7% (ZP704) and 0.13% (ZP434) to 7% (ZP704), respectively. Generally, the incidence of every particular fungus was higher in the late maize hybrid with a higher moisture content than in the medium-early hybrid with a lower moisture content.
AB  - Uzorci zrna dva hibrida kukuruza, srednje rani (ZP434) i kasni (ZP704), sakupljeni su u vreme berbe 2008. godine i analizirani na kontaminiranost gljivama. Zrna kukuruza zasejana su na hranljivu podlogu i razvijene gljive identifikovane su na osnovu morfoloških, makroskopskih i mikroskopskih osobina na krompirdekstroznoj (PDA) i sintetičkoj hranljivoj podlozi (SNA). Kod oba ispitivana hibrida vrste roda Fusarium su bile najzastupljenije sa prisustvom od 33,89% (ZP434) do 42% (ZP704). Ostali rodovi gljiva, Acremonium, Alternaria, Aspergillus, Chaetomium, Cladosporium, Mucor, Nigrospora, Penicillium, and Trichotecium, izolovani su od 0 do 41%. U rodu Fusarium identifikovane su četiri vrste, od kojih je vrsta F. verticillioides bila najzastupljenija sa 28,63% kod hibrida ZP434 i 30,5% kod hibrida ZP704. Vrsta F. graminearum bila je prisutna od 3% (ZP704) do 5% (ZP434), F. proliferatum od 0,13% (ZP434) do 7% (ZP704) i F. Subglutinans od 0,13% (ZP434) do 1,5% (ZP704). Uopšteno, ustanovljena je značajnija pojava svake pojedinačne vrste gljive kod kasnog hibrida sa višim sadržajem vlage u zrnu, nego kod srednje ranih hibrida sa nižim sadržajem vlage u zrnu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Occurrence of Fusarium species in maize grains for silage
T1  - Pojava Fusarium vrsta u zrnu kukuruza za silažu
VL  - 27
IS  - 3
SP  - 1235
EP  - 1240
DO  - 10.2298/BAH1103235K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Bijelić, Zorica and Mandić, Violeta and Stojanović, Lj.",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/385",
abstract = "Grain samples of two maize hybrids, medium early (ZP434) and late maturity (ZP704), collected during harvest in 2008 were investigated for contamination by fungi. Grains were plated on agar media and grown fungi were identified by morphological macroscopic and microscopic characteristics on potato-dextrose agar (PDA) and synthetic nutrient agar (SNA). Species of the genus Fusarium were the most common in both hybrids, and their presence amounted to 33.89% (ZP434) and 42% (ZP704). Other fungi of genera, Acremonium, Alternaria, Aspergillus, Chaetomium, Cladosporium, Nigrospora, Penicillium, Rhizopus and Trichotecium, were isolated from 0 to 41%. Four species belonging to the genus Fusarium were identified, of which the species F. verticillioides was the most common with 28.63% in ZP434 and 30.5% in ZP704 hybrids. The presence of F. graminearum, F. proliferatum and F. subglutinans ranged from 3% (ZP704) to 5% (ZP434), 0.13% (ZP434) to 7% (ZP704) and 0.13% (ZP434) to 7% (ZP704), respectively. Generally, the incidence of every particular fungus was higher in the late maize hybrid with a higher moisture content than in the medium-early hybrid with a lower moisture content., Uzorci zrna dva hibrida kukuruza, srednje rani (ZP434) i kasni (ZP704), sakupljeni su u vreme berbe 2008. godine i analizirani na kontaminiranost gljivama. Zrna kukuruza zasejana su na hranljivu podlogu i razvijene gljive identifikovane su na osnovu morfoloških, makroskopskih i mikroskopskih osobina na krompirdekstroznoj (PDA) i sintetičkoj hranljivoj podlozi (SNA). Kod oba ispitivana hibrida vrste roda Fusarium su bile najzastupljenije sa prisustvom od 33,89% (ZP434) do 42% (ZP704). Ostali rodovi gljiva, Acremonium, Alternaria, Aspergillus, Chaetomium, Cladosporium, Mucor, Nigrospora, Penicillium, and Trichotecium, izolovani su od 0 do 41%. U rodu Fusarium identifikovane su četiri vrste, od kojih je vrsta F. verticillioides bila najzastupljenija sa 28,63% kod hibrida ZP434 i 30,5% kod hibrida ZP704. Vrsta F. graminearum bila je prisutna od 3% (ZP704) do 5% (ZP434), F. proliferatum od 0,13% (ZP434) do 7% (ZP704) i F. Subglutinans od 0,13% (ZP434) do 1,5% (ZP704). Uopšteno, ustanovljena je značajnija pojava svake pojedinačne vrste gljive kod kasnog hibrida sa višim sadržajem vlage u zrnu, nego kod srednje ranih hibrida sa nižim sadržajem vlage u zrnu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Occurrence of Fusarium species in maize grains for silage, Pojava Fusarium vrsta u zrnu kukuruza za silažu",
volume = "27",
number = "3",
pages = "1235-1240",
doi = "10.2298/BAH1103235K"
}
Krnjaja, V., Lević, J., Stanković, S., Bijelić, Z., Mandić, V.,& Stojanović, Lj. (2011). Pojava Fusarium vrsta u zrnu kukuruza za silažu.
Biotechnology in Animal HusbandryInstitut za stočarstvo, Beograd., 27(3), 1235-1240.
https://doi.org/10.2298/BAH1103235K
Krnjaja V, Lević J, Stanković S, Bijelić Z, Mandić V, Stojanović L. Pojava Fusarium vrsta u zrnu kukuruza za silažu. Biotechnology in Animal Husbandry. 2011;27(3):1235-1240
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Bijelić Zorica, Mandić Violeta, Stojanović Lj., "Pojava Fusarium vrsta u zrnu kukuruza za silažu" 27, no. 3 (2011):1235-1240,
https://doi.org/10.2298/BAH1103235K .